光学学报
光學學報
광학학보
ACTA OPTICA SINICA
2009年
10期
2860-2865
,共6页
李兰权%魏华江%何博华%郭周义%杨洪钦%谢树森%陈雪梅%李力波%巫国勇%鲁建军
李蘭權%魏華江%何博華%郭週義%楊洪欽%謝樹森%陳雪梅%李力波%巫國勇%魯建軍
리란권%위화강%하박화%곽주의%양홍흠%사수삼%진설매%리력파%무국용%로건군
医用光学与生物技术%漫反射光谱%组织光学%紫外可见光分光光度计%积分球附件%胃癌粘膜组织
醫用光學與生物技術%漫反射光譜%組織光學%紫外可見光分光光度計%積分毬附件%胃癌粘膜組織
의용광학여생물기술%만반사광보%조직광학%자외가견광분광광도계%적분구부건%위암점막조직
medical optics and biotechnology%diffuse reflectance spectra%tissue optics%UV-visible spectrophotometer%integrating-spheire accessory%gastric mucosa tissue
采用氧合血红蛋白吸收带的平均漫反射光谱比率(R_(540)/R_(575))对离体的人正常胃、未分化胃腺癌、胃鳞状细胞癌和低分化胃腺癌的上皮粘膜组织进行鉴别诊断.实验采用带积分球附件的分光光度计测量组织样品在400 nm～600 nm的漫反射光谱.实验结果表明,在相同的波长范围内,人的未分化胃腺癌、胃鳞状细胞癌和低分化胃腺癌的上皮粘膜组织的漫反射光的强度较人正常胃的上皮粘膜组织的漫反射光强度要显著地低的,尤其是在氧合血红蛋白的吸收带540 nm和575 nm波长处,人正常胃、未分化胃腺癌、胃鳞状细胞癌和低分化胃腺癌的上皮粘膜组织的平均漫反射光谱比率分别是(94.25±2.4)%,(109±3.0)%,(1032±2.8)%和(98.6±2.6)%.
採用氧閤血紅蛋白吸收帶的平均漫反射光譜比率(R_(540)/R_(575))對離體的人正常胃、未分化胃腺癌、胃鱗狀細胞癌和低分化胃腺癌的上皮粘膜組織進行鑒彆診斷.實驗採用帶積分毬附件的分光光度計測量組織樣品在400 nm～600 nm的漫反射光譜.實驗結果錶明,在相同的波長範圍內,人的未分化胃腺癌、胃鱗狀細胞癌和低分化胃腺癌的上皮粘膜組織的漫反射光的彊度較人正常胃的上皮粘膜組織的漫反射光彊度要顯著地低的,尤其是在氧閤血紅蛋白的吸收帶540 nm和575 nm波長處,人正常胃、未分化胃腺癌、胃鱗狀細胞癌和低分化胃腺癌的上皮粘膜組織的平均漫反射光譜比率分彆是(94.25±2.4)%,(109±3.0)%,(1032±2.8)%和(98.6±2.6)%.
채용양합혈홍단백흡수대적평균만반사광보비솔(R_(540)/R_(575))대리체적인정상위、미분화위선암、위린상세포암화저분화위선암적상피점막조직진행감별진단.실험채용대적분구부건적분광광도계측량조직양품재400 nm～600 nm적만반사광보.실험결과표명,재상동적파장범위내,인적미분화위선암、위린상세포암화저분화위선암적상피점막조직적만반사광적강도교인정상위적상피점막조직적만반사광강도요현저지저적,우기시재양합혈홍단백적흡수대540 nm화575 nm파장처,인정상위、미분화위선암、위린상세포암화저분화위선암적상피점막조직적평균만반사광보비솔분별시(94.25±2.4)%,(109±3.0)%,(1032±2.8)%화(98.6±2.6)%.
Differential diagnosis of epithelial tissues of normal human gastric, undifferentiated gastric adenocarcinoma, gastric squamous cell carcinomas, and poorly differentiated gastric adenocarcinoma were studied using the mean diffuse reflectance spectral ratio (R_(540)/R_(575)) of the oxygenated hemoglobin absorption bands. Exvivo diffuse reflectance spectra in the 400 nm to 600nm region was measured from surgically removed gastric lesions using a spectrophotometer with an integrating sphere attachment. The results showed that the reflectance spectral intensity of the malignant tissues was lower than that of human normal gastric tissues and showed dips at 540 nm and 575 nm owing to absorption from oxygenated hemoglobin. Measurements carried out, the diffuse reflectance spectral ratio for epithelial tissues of normal human gastric, undifferentiated gastric adenocarcinoma, gastric squamous cell carcinomas, and poorly differentiated gastric adenocarcinoma were (94.2±2.4)%, (109±3.0)%, (103±2.8)% and (98.6±2.6)% respectively.
