CAJ | 학술논문

目的：探讨新生儿败血症患儿单核细胞CD14+/CD16+表达水平及其临床意义。方法：用流式细胞术测定血中单核细胞CD14+／CD16+表达水平，用放射酶联免疫吸附试验测定血中IL-6，IL-10和TNF-a水平，用Bactec-9120细菌培养系统进行血培养和药物敏感试验。结果：新生儿败血症组血中单核细胞CD14+／CD16+表达水平，IL-6，TNF-α水平显著高于正常新生儿组和非败血症组(P＜0.01)，而IL-10水平则显著低于正常新生儿组和非败血症组(P＜0.01)；非败血症组单核细胞CD14+／CD16+表达水平与正常组无显著性差异(P＞0.05)；败血症组单核细胞CD14+/CD16+表达水平与其IL-6，TNF-α水平呈显著正相关(P＜0.01)，与IL-10呈负相关(P＜0.01)；败血症组单核细胞CD14+／CD16+表达水平随病青好转而逐渐降低，病情危重时则持续高表达。结论：新生儿血中单核细胞CD14+/CD16+表达水平仅在败血症时显著增高，并随病情而变化，因此，它可能对败血症具有诊断意义，与败血症的预后有一定联系。
목적：탐토신생인패혈증환인단핵세포CD14+/CD16+표체수평급기림상의의。방법：용류식세포술측정혈중단핵세포CD14+／CD16+표체수평，용방사매련면역흡부시험측정혈중IL-6，IL-10화TNF-a수평，용Bactec-9120세균배양계통진행혈배양화약물민감시험。결과：신생인패혈증조혈중단핵세포CD14+／CD16+표체수평，IL-6，TNF-α수평현저고우정상신생인조화비패혈증조(P＜0.01)，이IL-10수평칙현저저우정상신생인조화비패혈증조(P＜0.01)；비패혈증조단핵세포CD14+／CD16+표체수평여정상조무현저성차이(P＞0.05)；패혈증조단핵세포CD14+/CD16+표체수평여기IL-6，TNF-α수평정현저정상관(P＜0.01)，여IL-10정부상관(P＜0.01)；패혈증조단핵세포CD14+／CD16+표체수평수병청호전이축점강저，병정위중시칙지속고표체。결론：신생인혈중단핵세포CD14+/CD16+표체수평부재패혈증시현저증고，병수병정이변화，인차，타가능대패혈증구유진단의의，여패혈증적예후유일정련계。
Objective:To investigate the expression of CD14+/CD16+ by monocytes in neonate septicenmia and its significance. Methods:The exprression of CD14+/CD16+ by monocytes in 124 neonates was analyzed by Flow cytometry, the levels of IL-6、IL-10 and TNF-α in bloo dwas measured by radioimmunoassay and bacteria culture and drug sensitive were tested by Bactec-9120 bacteria culture system Results: The expression levles of CD14 +/CD16 + by monocytes and the levels of IL-6 and TNF-α in the septicemia gnoup vere significantly higher than those in the normal group and non-septicemia group ( P ＜0.01).However, the levels of IL-10 was significantly lower in the septicemia group( P ＜0.01).The expression of CD14+/CD16+ by monocytes showed a positive correlation to the levels of IL-6 and TNF-α( P ＜0.01) and a nega-tive correlation to the level of IL-10( P ＜ 0.01) ;The expression of CD14+/CD16+ by monocytes changed with the condition of the sick neo-nates.Conchusion:The expression of CD14+/CD16+ by monocytes was increaseed in the neonates with septicemia and changed with the condi-tion of the sick neonates.Therefore,it could be useful for diagnosic and prognostic marker for neonate septicemia.