CAJ | 학술논문

在Fe_3O_4/Au微粒上固定乙酰胆碱酯酶(AChE),制得磁性复合粒子Fe_3O_4/Au/AChE.通过磁力将其吸附于涂覆了碳纳米管(CNTs)/纳米ZrO_2/普鲁士蓝(PB)/Nafion(Nf)复合膜的丝网印刷碳电极(SPCEs)表面,制得一次性有机磷农药(OPs)酶传感器.采用扫描电镜(SEM)、X射线荧光光谱(XRFS)表征传感器的制备过程,采用循环伏安法(CV)和示差脉冲伏安法(DPV)研究了传感器的电化学性质.利用OPs对AChE的抑制作用,以硫代乙酰胆碱(ATCh)为底物,对乐果进行了检测.在pH=7.5的0.1 mol/L硝酸钾溶液中,乐果浓度的对数与酶电极的抑制率(A)在1.0×10~(-6)～1.0×10~(-2 )mg/L间呈良好的线性关系,检测限为5.6×10~(-7 )mg/L,用于实际样品白菜检测时的添加回收率在88% ～105%之间,与气相色谱法(GC)所得结果一致.该传感器采用复合纳米粒子修饰电极表面,具有较高的比表面活性,响应迅速,检测限低;ZrO_2可特异性地富集样品中的OPs,磁性纳米颗粒包被AChE可实现磁场分离和电极表面更新,且具有高灵敏度、低样品量、一次使用可抛弃、便携式等特点,可用于蔬菜等农产品中痕量OPs的快速、简便、准确检测.
재Fe_3O_4/Au미립상고정을선담감지매(AChE),제득자성복합입자Fe_3O_4/Au/AChE.통과자력장기흡부우도복료탄납미관(CNTs)/납미ZrO_2/보로사람(PB)/Nafion(Nf)복합막적사망인쇄탄전겁(SPCEs)표면,제득일차성유궤린농약(OPs)매전감기.채용소묘전경(SEM)、X사선형광광보(XRFS)표정전감기적제비과정,채용순배복안법(CV)화시차맥충복안법(DPV)연구료전감기적전화학성질.이용OPs대AChE적억제작용,이류대을선담감(ATCh)위저물,대악과진행료검측.재pH=7.5적0.1 mol/L초산갑용액중,악과농도적대수여매전겁적억제솔(A)재1.0×10~(-6)～1.0×10~(-2 )mg/L간정량호적선성관계,검측한위5.6×10~(-7 )mg/L,용우실제양품백채검측시적첨가회수솔재88% ～105%지간,여기상색보법(GC)소득결과일치.해전감기채용복합납미입자수식전겁표면,구유교고적비표면활성,향응신속,검측한저;ZrO_2가특이성지부집양품중적OPs,자성납미과립포피AChE가실현자장분리화전겁표면경신,차구유고령민도、저양품량、일차사용가포기、편휴식등특점,가용우소채등농산품중흔량OPs적쾌속、간편、준학검측.
The composite magnetic particulate composed of Fe_3O_4/Au/acetylcholinesterase (AChE) was synthesized and was absorbed on the surface of screen-printed carbon electrodes (SPCEs) coated with carbon nanotubes(CNTs)/nano-ZrO_2/prussian blue (PB)/nafion (Nf)-membrane through magnet.A disposable organophosphorus pesticides (OPs) enzyme biosensor was developed for the determination of dimethoate.Different technologies were employed to study the construction process and electrochemical properties of the biosensor.In pH 7.5 0.1 mol/L KNO3 solution,the rate of inhibition (A) of the AChE by OPs was related linearly to the concentration of dimethoate in the range from 1.0×10~(-6) to 1.0×10~(-2 )mg/L with a detection limit of 5.6×10~(-7 )mg/L.The recovery was 88%-105% for chinese cabbage.The results of the analytical method were compaprable with that of gas chromatography method.The proposed biosensor has high surface activity due to the modification with composite nano-particles,rapid response,specific strong adsorption to OPs and was easily renew by removal of the magnet.It showed the merits of high sensitivity, disposabledesign,low consume of sample and portablity compared with other enzyme biosensors.This convenient,fast and sensitive voltammetric test method is suitable for the fast anlysis of OPs.