CAJ | 학술논문

目的：建立一种提取小肠上皮内淋巴细胞的方法，并对其功能进行初步研究。方法：采用机械分离法和Percoll非连续密度梯度离心法提取小鼠小肠上皮内淋巴细胞，经间接荧光染色后用流式细胞仪检测表型。应用乳酸脱氢酶法检测其自然杀伤功能。结果：应用该法可获得小鼠小肠上皮内淋巴细胞2×106～4×106/只鼠，活力测定＞95%。抗原表型中73.8%以上表达CD8分子，TCRγδ为43.8%。而且自然杀伤活性明显高于小鼠脾淋巴细胞。结论：表明采用该法提取小鼠小肠上皮内淋巴细胞有效。IEL具有自然杀伤活性。
목적：건립일충제취소장상피내림파세포적방법，병대기공능진행초보연구。방법：채용궤계분리법화Percoll비련속밀도제도리심법제취소서소장상피내림파세포，경간접형광염색후용류식세포의검측표형。응용유산탈경매법검측기자연살상공능。결과：응용해법가획득소서소장상피내림파세포2×106～4×106/지서，활력측정＞95%。항원표형중73.8%이상표체CD8분자，TCRγδ위43.8%。이차자연살상활성명현고우소서비림파세포。결론：표명채용해법제취소서소장상피내림파세포유효。IEL구유자연살상활성。
Objective:To extract a method for isolating murineintraepithelial lymphocy te and analyze its main function.Methods:Murine intestinal intraepithelial lymphocyte was isolated by a mechanical technique and Percoll d i scontinous density gradient centrigugation.Its surface antigens were measured by using indirect immunoflurescence.Its natural killing activity was examined by L DH method.Results:2×106～4×106iIELs/per mouse were a cquired by using this method,add its vitality＞95%.Of phenotypic antigen,more than 73. 8 % showed CD8+cell,TCRγδ 43.8%.IEL's natural killing activity was high er than PBMCs.Conclusion:This method is effective of isolating IELs.IELs has natural killing activity.