CAJ | 학술논문

选同一株系试管苗材料,在MS无激素培养基上切繁培养1周后,设置快繁、结薯、保存3个处理,从第2周到11周,每周统计单株生物产量、单株培养基下面的生物产量、单株培养基上面的生物产量,通过激素酶联试剂盒测定出吲哚乙酸、玉米素、脱落酸含量情况,并用数理统计方法建立各模式下内源激素与生物产量之间的线性相关方程,制订出一套试管苗快繁、结薯、保存的三区分管模式,为马铃薯细胞遗传分析、基因表达研究提供相关信息.
선동일주계시관묘재료,재MS무격소배양기상절번배양1주후,설치쾌번、결서、보존3개처리,종제2주도11주,매주통계단주생물산량、단주배양기하면적생물산량、단주배양기상면적생물산량,통과격소매련시제합측정출신타을산、옥미소、탈락산함량정황,병용수리통계방법건립각모식하내원격소여생물산량지간적선성상관방정,제정출일투시관묘쾌번、결서、보존적삼구분관모식,위마령서세포유전분석、기인표체연구제공상관신식.
In vitro seedling derived from one potato strain was incubated on non-hormone MS culture medium. Three experiments, including rapid reproduction, fruition and storage, were performed in two weeks. Biomasses of each individual plant inside and outside the culture medium and total plant were surveyed in each week from the second to the eleventh weeks. Level of indoleacetic acid (IAA), zeatin (ZA) and abscisic acid (ABA) were measured by enzyme-linked immunosorbent assay (ELISA), and were used to construct a linear correlation equation between endogenetic hormone and biomass. By which a three regions managing pattern of rapid reproduction, fruition and storage was worked out. Our conclusion could provide concerning information for cytogenetic analysis and genes expression of potato.