苏州医学院学报
囌州醫學院學報
소주의학원학보
JACTA ACADEMIAE MEDICINAE SUZHOU
2001年
2期
228-230
,共3页
卜曙阳%黄强%周丽英%孙立军%孙志方
蔔曙暘%黃彊%週麗英%孫立軍%孫誌方
복서양%황강%주려영%손립군%손지방
视神经%培养%细胞%小鼠
視神經%培養%細胞%小鼠
시신경%배양%세포%소서
目的 介绍一种快速原代培养、分离、纯化视神经星形胶质细胞的方法。方法 对P2期小鼠的视神经进行组织块原代培养，振荡法分离纯化，形态学观察，免疫组织化学染色。结果 培养的星形细胞纯度高，GFAP染色显示98%阳性。结论 组织块原代培养，振荡法分离纯化视神经的星形胶质细胞，对细胞的损伤小、纯度高，是快速得到该细胞的理想途径。
目的 介紹一種快速原代培養、分離、純化視神經星形膠質細胞的方法。方法 對P2期小鼠的視神經進行組織塊原代培養，振盪法分離純化，形態學觀察，免疫組織化學染色。結果 培養的星形細胞純度高，GFAP染色顯示98%暘性。結論 組織塊原代培養，振盪法分離純化視神經的星形膠質細胞，對細胞的損傷小、純度高，是快速得到該細胞的理想途徑。
목적 개소일충쾌속원대배양、분리、순화시신경성형효질세포적방법。방법 대P2기소서적시신경진행조직괴원대배양，진탕법분리순화，형태학관찰，면역조직화학염색。결과 배양적성형세포순도고，GFAP염색현시98%양성。결론 조직괴원대배양，진탕법분리순화시신경적성형효질세포，대세포적손상소、순도고，시쾌속득도해세포적이상도경。
Objective To introduce a method for condition primary culture of astrocyte of optic nerve.Methods Conditioned medium was made of GGF-BF ,and astrocyte of optic nerve was cultured and purified by shaking.Identification was made by means of microscope observation and immunohistochemistry.Results Astrocyte of rat optic nerve was cultured and purified,which showed positive reaction to glial fibrillary acidic protein(GFAP). Conclusion Conditioned primary culture of rat optic nerve, purified through shaking,is a reliable and quick method.
