CAJ | 학술논문

目的：探讨深低温保存对脐血长期培养起始细胞(Long term culture initial cell,LTC-IC)含量的影响。方法：利用6%羟乙基淀粉法分离脐血单个核细胞，采用基质细胞直接接触培养系统和CFU-GM检测方法检测新鲜脐血和深低温保存的脐血中长期培养起始细胞和造血祖细胞。结果：脐血深低温保存复苏后，CFU-GM由97±5个/1×105 MNC减少到84±13个/1×105 MNC，回收率为(83±12)%；LTC-IC 87±20个/1×105MNC减少到68±33个/1×105MNC，回收率为(76±33)%。结论：深低温保存后，脐血中LTC-IC的含量减少，但脐血仍保存大部分造血功能。
목적：탐토심저온보존대제혈장기배양기시세포(Long term culture initial cell,LTC-IC)함량적영향。방법：이용6%간을기정분법분리제혈단개핵세포，채용기질세포직접접촉배양계통화CFU-GM검측방법검측신선제혈화심저온보존적제혈중장기배양기시세포화조혈조세포。결과：제혈심저온보존복소후，CFU-GM유97±5개/1×105 MNC감소도84±13개/1×105 MNC，회수솔위(83±12)%；LTC-IC 87±20개/1×105MNC감소도68±33개/1×105MNC，회수솔위(76±33)%。결론：심저온보존후，제혈중LTC-IC적함량감소，단제혈잉보존대부분조혈공능。
Objective:To explore the effect of cryopreservation on LTC-IC counts of human umbilical cord blood.Method:Placental cord blood mononuclear cells were separeted by 6% hetastarch and cryopreserved in liquid nitrogen with 10% DMSO. LTC-IC count and CFU-GM were detected by stromal-contact culture system and CFU-GM culture system.Result:After thawing,CFU-GM decreased from 97±5/1×105 MNC to 84±13/1×105 MNC,the recovery was (83±12)%; the number of LTC-IC clone also decreased from 87±20/1×105MNC to 68±33/1×105 MNC,the recovery was (76±33)%.Conclusion:Cryopreservation could bring into some LTC-IC lost,but have no influence on their major hematopoietic potentiality.