CAJ | 학술논문

提出了一种地衣芽孢杆菌β-甘露聚糖酶制备的工艺。结果表明：在设定的操作条件下，6.6L自控罐发酵酶活可达260u/mL。发酵液用AlCl3与壳聚糖絮凝预处理后，絮凝体喷雾干燥可制成900u/g的酶粉，适合饲料和造纸工业使用；絮凝上清液先以平均截留分子质量5万的中空纤维聚砜膜分离，透过液再用平均截留分子质量1万的中空纤维聚砜膜浓缩后，喷雾干燥可制成11000u/g的酶粉，适合食品和医药工业使用。以上工艺酶活总收率88％，并易于工业放大。
제출료일충지의아포간균β-감로취당매제비적공예。결과표명：재설정적조작조건하，6.6L자공관발효매활가체260u/mL。발효액용AlCl3여각취당서응예처리후，서응체분무간조가제성900u/g적매분，괄합사료화조지공업사용；서응상청액선이평균절류분자질량5만적중공섬유취풍막분리，투과액재용평균절류분자질량1만적중공섬유취풍막농축후，분무간조가제성11000u/g적매분，괄합식품화의약공업사용。이상공예매활총수솔88％，병역우공업방대。
A process has been developed for the preparation of β-mannanase from Bacillus licheniformis. The enzyme activity was 260 u/mL when incubated in a 6.6L fermentor under the designed conditions. After pretreatment of culture broth using flocculants of AlCl3 and chitosan,the floc was spray-dried (900 u/g) for use in feed or pulp industries, while the supernatant was fractionated by polysulfone membrane and then concentrated by polysulfone membrane. The final product was spray-dried (11 000u/g) for use in food or pharmaceutical industries. The proposed process resulted in the total yield of β-mannanase activity of 88 % and is easily scale-up.