CAJ | 학술논문

目的了解抗病毒1号单味中草药对流感病毒A 3的抑制作用.方法采用细胞培养技术,以病毒唑为阳性对照药,观察抗病毒 1号对流感病毒的抑制作用.结果抗病毒1号半数中毒浓度(TC50) 为60.53 mg*ml-1;治疗和预防给药方式均有抗流感病毒作用,其抗流感病毒A3的半数有效浓度(EC50)分别为5.14 mg*ml-1和5.20 mg*ml-1;治疗指数 (TI)分别为11.78和11.65;抗病毒1号对流感病毒A3的抑制作用存在着明显的量效反应关系(P<0.01).结论抗病毒1号在MDCK细胞中对流感病毒A3有抑制作用.
목적료해항병독1호단미중초약대류감병독A 3적억제작용.방법채용세포배양기술,이병독서위양성대조약,관찰항병독 1호대류감병독적억제작용.결과항병독1호반수중독농도(TC50) 위60.53 mg*ml-1;치료화예방급약방식균유항류감병독작용,기항류감병독A3적반수유효농도(EC50)분별위5.14 mg*ml-1화5.20 mg*ml-1;치료지수 (TI)분별위11.78화11.65;항병독1호대류감병독A3적억제작용존재착명현적량효반응관계(P<0.01).결론항병독1호재MDCK세포중대류감병독A3유억제작용.
Objective To observe the anti-influenza effect of a Chinese medicinal herb-Antiviral Agent No.1. Methods To study t he anti-viral effect of Antiviral Agent No.1 by means of the technique of cel l culture and using Ribavirin as a positive control. Results In MDC K cu lture, Antiviral Agent No.1 was found to be a potential inhibitor of influenza A 3 virus in a concentration-dependent manner, with a TC50 of 60.53 mg*m l-1. When drug was added 2 hours post virus infection, the EC50(TI) was 5.14 mg*ml-1(11.78); while drug was added 2 hours before infection, t he EC50(TI) was 5.20 mg*ml-1(11.65). Conclusions Antiv iral Agent No.1 had a significant anti-influenza effect on type A3 in MDCK.