CAJ | 학술논문

采用人SRY基因的一段保守序列的引物，通过PCR在雄性赤麂中扩增出了赤麂SRY 基因的特异片段,通过DNA斑点杂交证实其扩增产物与人SRY基因有着较高的同源性。将PCR产物克隆到载体pGEM T Vector中,转化DH5α菌,经与人SRY基因探针进行菌落杂交筛选出赤麂SRY基因的阳性克隆,并对其进行了测序.将其序列与基因库中录入的所有偶蹄目动物的SRY基因序列进行同源性比较，用UPGMA法构建了其系统进化树，从分类和进化上对赤麂SRY基因进行分析
채용인SRY기인적일단보수서렬적인물，통과PCR재웅성적궤중확증출료적궤SRY 기인적특이편단,통과DNA반점잡교증실기확증산물여인SRY기인유착교고적동원성。장PCR산물극륭도재체pGEM T Vector중,전화DH5α균,경여인SRY기인탐침진행균락잡교사선출적궤SRY기인적양성극륭,병대기진행료측서.장기서렬여기인고중록입적소유우제목동물적SRY기인서렬진행동원성비교，용UPGMA법구건료기계통진화수，종분류화진화상대적궤SRY기인진행분석
With human SRY gene primers of the conserved region, the SRY gene fragment of M.m.vaginalis homologous to human SRY was amplified by PCR. In male M.m.vaginalis, a gene fragment of 221 bp homologous to human SRY was identified by DNA dot hybridization. By ligating pGEMT Vector with the PCR products of SRY gene of M.m.vaginalis and transforming the bacteria DH5α and clony hybridization with human SRY probe, the positive clony with M.m.vaginalis Sry gene fragment was identified gene, the SRY fragment of this clony was sequenced. The sequences identities of SRY gene fragments of all animals of Artiodactyla available from GenBank were compared with the M.m.vaginalis SRY gene fragment. The phylogenetic tree was contructed and analyzed by UPGMA method and it was studied at the level of taxonomics and evolution.