CAJ | 학술논문

目的探讨pDL121的1.0kb017株问号赖型钩端螺旋体DNA片段可否作为钩体重组疫苗广谱保护性抗原的候选。方法采用SDS-PAGE制备23kDa蛋白，免疫日本大耳兔制备抗23kDa抗血清，Western blot鉴定其免疫原性。结果 23kDa重组抗原兔抗血清可识别pDL121体外表达23kDa抗原产物和问号赖型钩端螺旋体017株超声抗原成份；用Westem blot鉴定抗血清效价，其抗体滴度为1／12800；用pDL121重组质粒主动免疫豚鼠，可使豚鼠抵抗强毒力株钩端螺旋体攻击，表现出免疫保护作用。结论该重组23kDa抗原有良好的免疫原性，可能是赖型钩体017株的保护性抗原。
목적탐토pDL121적1.0kb017주문호뢰형구단라선체DNA편단가부작위구체중조역묘엄보보호성항원적후선。방법채용SDS-PAGE제비23kDa단백，면역일본대이토제비항23kDa항혈청，Western blot감정기면역원성。결과 23kDa중조항원토항혈청가식별pDL121체외표체23kDa항원산물화문호뢰형구단라선체017주초성항원성빈；용Westem blot감정항혈청효개，기항체적도위1／12800；용pDL121중조질립주동면역돈서，가사돈서저항강독력주구단라선체공격，표현출면역보호작용。결론해중조23kDa항원유량호적면역원성，가능시뢰형구체017주적보호성항원。
In order to test whether the 23kDa protein could be the candidate for new generation vaccines against leptospirosis for the developing world,the isolated 23kDa protein band was cut and injected twice into rabbits to raise anti-23kDa serum. What is more,the protection of E. coli lysate of pDL121 on guinea pigs was done. The results showed that the anti23kDa serum could recognize the sonicated antigen of L. interrogans serovar lai strain 017 and 23kDa protein expressed in pDL121 and the tilter of the antiserum were very high,approximately 1/12800. Injection of the E. coli lysate of pDL121 with Freund's adjuvant into guinea pigs resulted in some protection of the animal against the challenge with strain 017. This indicated that 23kDa had good imunogenicity and could serve as a candidate for protective antigen of L. interrogans.