CAJ | 학술논문

为了培育适合青海高原种植的春小麦糯性新品系,利用DNA分子标记技术检测了80株青春533×糯麦1号F_2代材料的Wx基因缺失情况,同时测定了不同Wx基因缺失类型F_2代材料的小麦籽粒直链淀粉含量.结果表明,在80株F_2代分离群体材料中,单缺失Wx-A1或Wx-B1或Wx-D1基因的材料分别为8份、11份、9份;同时缺失Wx-A1和Wx-D1基因的材料为10份,同时缺失Wx-B1和Wx-D1基因的材料为12份,同时缺失Wx-A1和Wx-B1基因的材料为9份;Wx-A1、Wx-B1和Wx-D1基因全部缺失的材料为8份.Wx基因的缺失数量与直链淀粉含量的高低有密切关系,缺失1个Wx基因(部分糯小麦),直链淀粉含量比对照(青春533)降低5.28%～11.35%;缺失2个Wx基因(部分糯小麦),直链淀粉含量比对照降低10.73%～19.81%;缺失3个基因(糯小麦)的直链淀粉含量为0.97%,比对照降低30.53%.高原春小麦与糯性小麦杂交的F_2代直链淀粉含量下降幅度要比前人所研究的材料下降幅度大,并且Wx-B1单缺失引起直链淀粉含量下降(11.35%)比Wx-A1和Wx-D1双缺失所引起的下降幅度要大(10.73%).
위료배육괄합청해고원충식적춘소맥나성신품계,이용DNA분자표기기술검측료80주청춘533×나맥1호F_2대재료적Wx기인결실정황,동시측정료불동Wx기인결실류형F_2대재료적소맥자립직련정분함량.결과표명,재80주F_2대분리군체재료중,단결실Wx-A1혹Wx-B1혹Wx-D1기인적재료분별위8빈、11빈、9빈;동시결실Wx-A1화Wx-D1기인적재료위10빈,동시결실Wx-B1화Wx-D1기인적재료위12빈,동시결실Wx-A1화Wx-B1기인적재료위9빈;Wx-A1、Wx-B1화Wx-D1기인전부결실적재료위8빈.Wx기인적결실수량여직련정분함량적고저유밀절관계,결실1개Wx기인(부분나소맥),직련정분함량비대조(청춘533)강저5.28%～11.35%;결실2개Wx기인(부분나소맥),직련정분함량비대조강저10.73%～19.81%;결실3개기인(나소맥)적직련정분함량위0.97%,비대조강저30.53%.고원춘소맥여나성소맥잡교적F_2대직련정분함량하강폭도요비전인소연구적재료하강폭도대,병차Wx-B1단결실인기직련정분함량하강(11.35%)비Wx-A1화Wx-D1쌍결실소인기적하강폭도요대(10.73%).
In order to breed new waxy spring wheat lines that adapt to the environment of Qinghai Plateau,80 F_2 segregation plants of Qingchun 533×Nuomai No1 were harvested, four PCR-based markers were used to detect the genotypes of these plants,amylose content determination of these progeny plants with different genotypes was also conducted. The number of plants that lacked of Wx-A1、Wx-B1、Wx-D1 were 8、11、9 respectively;10 plants lacked both Wx-A1 and Wx-D1,12 plants lacked both Wx-B1 and Wx-D1, 9 plants lacked both Wx-A1 and Wx-B1, 8 plants lacked the three Wx genes.The amylose content was 5.28%～11.35% lower than that of Qingchun 533 while one Wx gene was absent. When two Wx genes were absent in progeny plants,the amylose content was 10.73%～19.81% lower than that of Qingchun 533.The progeny plants with absence of three Wx genes had the lowest amylose content (0.97%). The results of this study were subtly different with the previous report, the decrease of amylose content was more acute when there was an absence of Wx gene (genes) in progeny plants, decrease of amylose content in progeny plants with absence of Wx-B1 was more acute than that of progeny plants with absence of Wx-A1 and Wx-D1.