CAJ | 학술논문

纳秒瞬态拉曼光谱技术是研究分子结构变化超快动态过程的重要实验手段之一.而肌红蛋白(Mb)与小分子配体的结合过程一直是人们研究的焦点.本文旨在利用纳秒瞬态拉曼光谱技术研究小分子配体NO与肌红蛋白结合的动力学过程.通过考察MbNO光解后产物脱氧肌红蛋白(DeoxyMb)与反应物MbNO的V_4特征振动峰的强度比值随激光激发功率的变化,阐述了利用纳秒瞬态拉曼光谱技术研究MbNO体系中NO与DeoxyMb结合过程的可行性.利用纳秒瞬态拉曼光谱技术,获得了与皮秒时间分辨拉曼和皮秒时间分辨吸收相一致的结合动力学实验结果.为研究其它复杂体系的超快结合动力学过程提供了一种新的思路.
납초순태랍만광보기술시연구분자결구변화초쾌동태과정적중요실험수단지일.이기홍단백(Mb)여소분자배체적결합과정일직시인문연구적초점.본문지재이용납초순태랍만광보기술연구소분자배체NO여기홍단백결합적동역학과정.통과고찰MbNO광해후산물탈양기홍단백(DeoxyMb)여반응물MbNO적V_4특정진동봉적강도비치수격광격발공솔적변화,천술료이용납초순태랍만광보기술연구MbNO체계중NO여DeoxyMb결합과정적가행성.이용납초순태랍만광보기술,획득료여피초시간분변랍만화피초시간분변흡수상일치적결합동역학실험결과.위연구기타복잡체계적초쾌결합동역학과정제공료일충신적사로.
Nanosecond transient Raman spectroscopy is a Widely used technique to investigate ultrafast structural dynamics in molecules.We studied the rebinding kinetics between deoxymyoglobin (DeoxyMb) and NO using nanosecond transient Raman spectroscopy.By monitoring the ratio between the intensity of the V_4 vibrational mode in the photoproduct(DeoxyMb) and in the reactant(MbNO) at different incident laser fluxes,we obtained the recombination kinetics between DeoxyMb and NO in MbNO.For comparison.the kinetics of NO rebinding to DeoxyMb in MbNO was also studied using picosecond time-resolved Raman and absorption experiments.The kinetics results obtained With nanosecond transient Raman were consistent with those obtained by picosecond time-resolved Raman and absorption experiments.