CAJ | 학술논문

WSSV可经注射、投喂或共居感染克氏螯虾和日本沼虾，病毒感染的组织匀浆物注射后发病很快，但蔗糖介质纯化的WSSV注射后发病较慢，Ficoll-400介质纯化的WSSV注射后发病时间介于前二者之间.中国对虾细胞内WSSV一般每个囊膜只含一个核衣壳，但此毒种经克氏螯虾反复增殖后，多粒包埋的病毒粒子明显增多.从病虾的每克鳃可提出约0.66mg的WSSV.含WSSV的血淋巴或组织匀浆液感染8种昆虫细胞后，传代2次仍未观察到病毒在其中复制.
WSSV가경주사、투위혹공거감염극씨오하화일본소하，병독감염적조직균장물주사후발병흔쾌，단자당개질순화적WSSV주사후발병교만，Ficoll-400개질순화적WSSV주사후발병시간개우전이자지간.중국대하세포내WSSV일반매개낭막지함일개핵의각，단차독충경극씨오하반복증식후，다립포매적병독입자명현증다.종병하적매극새가제출약0.66mg적WSSV.함WSSV적혈림파혹조직균장액감염8충곤충세포후，전대2차잉미관찰도병독재기중복제.
White spot syndrome virus infected Cambarus clarkii andMacrobrachium nipponensis by the means of injection, feeding or co-habitation. The period of this disease was short and the mortality was high. The inoculum was prepared from gill, pereiopod or tail of diseased shrimps by slow centrifugation could cause injected shrimps to die fast, but WSSV purified by sugar of Ficoll-400 cushion centrifugation made shrimps die slowly. Only few viruses whose envelope included two or nucleocapsids existed in cells of Penaeus chinesis, but a lot of viruses whose envelop included two or more nucleocapsids were observed in cells of Cambarus clarkii. The weight of WSSV per gram dried gill was 0.66 mg. The infection of eight insect cells with wssv was not successful.