CAJ | 학술논문

目的:运用基因表达谱芯片技术对肿瘤转移相关基因的表达谱进行研究.方法:对临床切除的肝癌和胰腺癌组织及相应的对照正常组织进行总RNA抽提,纯化后的mRNA进行逆转录制备杂交探针 ,应用BioDoor4096和BioDoor12800的全长基因DNA芯片筛选与肝癌和胰腺癌转移相关的基因 .杂交后的信号用ScanArray3000扫描,结果用ImaGene3.0软件分析.结果:对肝癌和胰腺癌基因表达谱进行分析,发现两种肿瘤中存在相同的差异表达基因,其中有15条基因与肝癌、胰腺癌转移密切相关.结论:通过对肝癌、胰腺癌的肿瘤表达谱芯片研究,尤其是与转移相关基因的分析,对了解肿瘤的发病机制和转移发生情况及其肿瘤的预后有重要意义.
목적:운용기인표체보심편기술대종류전이상관기인적표체보진행연구.방법:대림상절제적간암화이선암조직급상응적대조정상조직진행총RNA추제,순화후적mRNA진행역전록제비잡교탐침 ,응용BioDoor4096화BioDoor12800적전장기인DNA심편사선여간암화이선암전이상관적기인 .잡교후적신호용ScanArray3000소묘,결과용ImaGene3.0연건분석.결과:대간암화이선암기인표체보진행분석,발현량충종류중존재상동적차이표체기인,기중유15조기인여간암、이선암전이밀절상관.결론:통과대간암、이선암적종류표체보심편연구,우기시여전이상관기인적분석,대료해종류적발병궤제화전이발생정황급기종류적예후유중요의의.
Objective:To understand the molecular pat hophysiology of hepatocellular carcinoma and pancreatic cancer.Methods: We studied novel gene expression by cDNA microarray method. The PCR pro ducts of 4 096 genes and 12 800 gene were spotted onto a kind of chemical-mater ial-coated-glass slide in array. Both the mRNAs from 5 cases of hepatocellular carcinoma and 3 cases of pancreatic cancer were reversely transcribed to cDNAs with the incorporation of fluorescent-labeled dUTP to prepare the hybridization probes. After hybridization, BioDoor4096 and BioDoor12800 cDNA microarray were scanned for the fluorescent intensity. Tumor invasion-related gene expression w as screened through the analysis of difference in gene expression profile.Results:Among 4 096 and 12 800 target genes, there were 15 genes who se expression level differed from normal and carcinoma tissues. Therefore, they might be associated with metastasis.Conclusion:Further analysis of these differentially expressed metastasis-associated genes will be helpful for understanding the molecular mechanism of malignant carcinoma.