中山大学学报（医学科学版）
中山大學學報（醫學科學版）
중산대학학보（의학과학판）
JOURNAL OF SUN YAT-SEN UNIVERSITY(MEDICAL SCIENCES)
2010年
1期
45-49
,共5页
王丁丁%于强%陈子林%柳夏林
王丁丁%于彊%陳子林%柳夏林
왕정정%우강%진자림%류하림
神经生长因子(NGF)%人视网膜血管内皮细胞(HRCEC)%TrkA阻断剂K252a
神經生長因子(NGF)%人視網膜血管內皮細胞(HRCEC)%TrkA阻斷劑K252a
신경생장인자(NGF)%인시망막혈관내피세포(HRCEC)%TrkA조단제K252a
nerve growth factor (NGF)%human retinal vascular endothelial cells (HRCEC)%TrkA inhibitor (K252a)
[目的]观察神经生长因子(NGF)及TrKa阻断剂K252a对体外培养的人视网膜血管内皮细胞(HRCEC)增殖的影响.[方法]MTT法检测不同因子(NGF各浓度组、NGF+K252a各浓度组、bFGF组、bFGF+K252a组和正常培养液组)对正常和缺氧条件下培养的HRCEC的影响.[结果]随NGF浓度增加(20、50、100 ng/ml),HRCEC细胞数目明显增多(正常氧浓度NGF组分别为0.254±0.033、0.696±0.029、1.136±0.051;缺氧条件相应各组为0.422±0.036、0.798±0.044、1.376±0.052,P均<0.05).随K252a浓度增加(50、1000、200 nmol/L),NGF+K252a组正常氧浓度时分别为0.864±0.067、0.496±0.025、0.202±0.078,缺氧条件时相应为1.042±0.047、0.700±0.065、0.401±0.078,较同浓度NGF(100 ng/ml)组HRCEC细胞数目减少(P<0.05),随K252a浓度增加HRCEC细胞数目减少趋势愈加显著(P均<0.05).[结论]NGF可促进HRCEC的增殖,该作用可被特异性TrkA阻断剂K252a所阻断.
[目的]觀察神經生長因子(NGF)及TrKa阻斷劑K252a對體外培養的人視網膜血管內皮細胞(HRCEC)增殖的影響.[方法]MTT法檢測不同因子(NGF各濃度組、NGF+K252a各濃度組、bFGF組、bFGF+K252a組和正常培養液組)對正常和缺氧條件下培養的HRCEC的影響.[結果]隨NGF濃度增加(20、50、100 ng/ml),HRCEC細胞數目明顯增多(正常氧濃度NGF組分彆為0.254±0.033、0.696±0.029、1.136±0.051;缺氧條件相應各組為0.422±0.036、0.798±0.044、1.376±0.052,P均<0.05).隨K252a濃度增加(50、1000、200 nmol/L),NGF+K252a組正常氧濃度時分彆為0.864±0.067、0.496±0.025、0.202±0.078,缺氧條件時相應為1.042±0.047、0.700±0.065、0.401±0.078,較同濃度NGF(100 ng/ml)組HRCEC細胞數目減少(P<0.05),隨K252a濃度增加HRCEC細胞數目減少趨勢愈加顯著(P均<0.05).[結論]NGF可促進HRCEC的增殖,該作用可被特異性TrkA阻斷劑K252a所阻斷.
[목적]관찰신경생장인자(NGF)급TrKa조단제K252a대체외배양적인시망막혈관내피세포(HRCEC)증식적영향.[방법]MTT법검측불동인자(NGF각농도조、NGF+K252a각농도조、bFGF조、bFGF+K252a조화정상배양액조)대정상화결양조건하배양적HRCEC적영향.[결과]수NGF농도증가(20、50、100 ng/ml),HRCEC세포수목명현증다(정상양농도NGF조분별위0.254±0.033、0.696±0.029、1.136±0.051;결양조건상응각조위0.422±0.036、0.798±0.044、1.376±0.052,P균<0.05).수K252a농도증가(50、1000、200 nmol/L),NGF+K252a조정상양농도시분별위0.864±0.067、0.496±0.025、0.202±0.078,결양조건시상응위1.042±0.047、0.700±0.065、0.401±0.078,교동농도NGF(100 ng/ml)조HRCEC세포수목감소(P<0.05),수K252a농도증가HRCEC세포수목감소추세유가현저(P균<0.05).[결론]NGF가촉진HRCEC적증식,해작용가피특이성TrkA조단제K252a소조단.
[Objective] To observe NGF on cultured human retinal vascular endothelial cells (HRCEC) proliferation. [Methods] The MTT assay was used to analyze the impact of culture HRCEC on different factors (NGF concentration groups, NGF + K252a concentration groups, bFGF group, bFGF + K252a groups, the normal culture medium groups) in normal and hypoxic condition. [Results] With the increase of NGF concentration (20,50,100 ng/mL), HRCEC significantly increased (normal condition: 0.254±0.033,0.696±0.029, 1.136±0.051; hypoxic condition: 0.422±0.036, 0.798±0.044, 1.376±0.052, P< 0.05). Compared NGF + K252a group with the same concentration of NGF (100 ng/ml) group, HRCEC reduced (P<0.05), with increasing the concentration of K252a (50,100,200 nmol/L), the trend of HRCEC decreasing is become more significant (normal condition:0.864±0.067, 0.496±0.025, 0.202±0.078; hypoxic condition:K252a 1.042±0.047,0.700±0.065, 0.401±0.078, P<0.05). [Conclusion] NGF can promote the proliferation of HRCEC, the effect could be specifically blocked by TrkA inhibitor K252a.
