CAJ | 학술논문

目的:探讨自然流产绒毛外滋养细胞(Extravillous trophoblast cells,EVTs)侵袭相关基因表达变化.方法:利用U133 plus2.0芯片对自然流产绒毛和正常绒毛组织中EVTs的基因表达谱进行检测,并用生物信息学方法对检测结果进行分析.结果:与正常EVTs相比,在自然流产EVTs中,发现显著上调基因219个,下调基因293个,其功能主要涉及细胞粘附、免疫应答、代谢等.结论:与滋养层细胞细胞粘附、免疫应答、水解酶及凋亡等相关基因的差异表达可能与滋养细胞侵袭行为变化及自然流产有关.
목적:탐토자연유산융모외자양세포(Extravillous trophoblast cells,EVTs)침습상관기인표체변화.방법:이용U133 plus2.0심편대자연유산융모화정상융모조직중EVTs적기인표체보진행검측,병용생물신식학방법대검측결과진행분석.결과:여정상EVTs상비,재자연유산EVTs중,발현현저상조기인219개,하조기인293개,기공능주요섭급세포점부、면역응답、대사등.결론:여자양층세포세포점부、면역응답、수해매급조망등상관기인적차이표체가능여자양세포침습행위변화급자연유산유관.
Objective: To investigate the expression changes of invasion-relevant genes in spontaneous abortion Extravillous trophoblast ceils (EVTs).Methods: The U133 plus2.0 gene chips were used to analyze gene expression profiles in spontaneous EVTs and normal EVTs.The results were analyzed by bioinformatics methods.Results: Compared with normal EVTs,219 and 293 genes in spontaneous abortion EVTs were up-regulated and down-regulated respectively. These genes were mainly associated with signal transduction, apoptosis,metabolism,immune response,etc.Conclusion: Differential expression of genes related with apoptosis,immune response. hydratase,cell adhesion may play a role in the EVTs'abnormal invasion and spontaneous abortion.