CAJ | 학술논문

为寻求一种较好的显示肥大细胞的方法,采用硫堇染色法和硫堇-甲苯胺蓝染色法分别对用不同固定液固定的兔肠系膜中肥大细胞进行染色.结果显示:Carnoy氏液固定,硫堇染色可清楚显示肥大细胞,背景干扰小,阳性细胞多且可见肥大细胞的脱颗粒现象;硫堇-甲苯胺蓝法虽然也可显示肥大细胞,但背景干扰大,不利于对肥大细胞的观察;甲醛-乙醇固定,2种染色方法都可清楚地显示肥大细胞,但阳性细胞较少;中性甲醛则完全阻断肥大细胞的着色,2种染色方法都不能显示肥大细胞.兔肠系膜肥大细胞有沿血管分布的趋势.研究结果表明:Carnoy氏液及硫堇染液分别是兔肠系膜优良的固定液和染色液,可清楚显示肥大细胞.
위심구일충교호적현시비대세포적방법,채용류근염색법화류근-갑분알람염색법분별대용불동고정액고정적토장계막중비대세포진행염색.결과현시:Carnoy씨액고정,류근염색가청초현시비대세포,배경간우소,양성세포다차가견비대세포적탈과립현상;류근-갑분알람법수연야가현시비대세포,단배경간우대,불리우대비대세포적관찰;갑철-을순고정,2충염색방법도가청초지현시비대세포,단양성세포교소;중성갑철칙완전조단비대세포적착색,2충염색방법도불능현시비대세포.토장계막비대세포유연혈관분포적추세.연구결과표명:Carnoy씨액급류근염액분별시토장계막우량적고정액화염색액,가청초현시비대세포.
In the present study,rabbit mesentery was fixed in three different fixatives and stained with thionine and thionine-toluidine blue respectively in order to find a good method to display mast cells.The results showed that lots of positive mast cells with light background could be presented clearly by Carnoy fixative and thionine staining.In addition,degranulation of some mast cells could be observed clearly.However,it was difficult to observe positive mast cells by Carnoy fixative and thionine-toluidine blue staining because of high interferential background.Only a little positive mast cells could be observed through formaldehyde-alcohol fixative and the two staining methods.Neutral formalin blocked the staining of mast cells completely,for neither of the staining methods could display mast cells.Most of the positive mast cells were distributed along the blood vessels.The results demonstrated that Carnoy and thionine were superordinary fixative and staining solution for clearly displaying mast cells of rabbit mesentery,respectively.