CAJ | 학술논문

目的:研究通塞脉片(当归、党参、黄芪、石斛、川芎、金银花、牛漆、甘草)(TSM)对大鼠实验性动脉粥样硬化内皮细胞的影响.方法:50只♂性wistar大鼠,随机分为空白组、模型组、辛伐他汀组、TSM高剂量和低剂量组,除空白对照组给以普通饲料喂养外,其他组均饲以高脂饲料及维生素D_3,建立食饵性动脉粥样硬化模型.12周后检测外周血循环内皮细胞(CEC)、血管紧张素Ⅱ(AngⅡ)、电镜观察动脉血管形态.结果:模型组大鼠CEC明显增加,AngⅡ含量显著升高,TSM能够减少CEC,降低AngⅡ含量.透射电镜观察模型组大鼠主动脉内皮严重受损;TSM组大鼠主动脉内皮细胞扁平形态基本完整,内弹性膜平整厚度均匀,损伤明显减轻.结论:TSM通过减少CEC数量、降低AngⅡ水平,达到保护大鼠实验性动脉粥样硬化模型内皮细胞的作用.
목적:연구통새맥편(당귀、당삼、황기、석곡、천궁、금은화、우칠、감초)(TSM)대대서실험성동맥죽양경화내피세포적영향.방법:50지♂성wistar대서,수궤분위공백조、모형조、신벌타정조、TSM고제량화저제량조,제공백대조조급이보통사료위양외,기타조균사이고지사료급유생소D_3,건립식이성동맥죽양경화모형.12주후검측외주혈순배내피세포(CEC)、혈관긴장소Ⅱ(AngⅡ)、전경관찰동맥혈관형태.결과:모형조대서CEC명현증가,AngⅡ함량현저승고,TSM능구감소CEC,강저AngⅡ함량.투사전경관찰모형조대서주동맥내피엄중수손;TSM조대서주동맥내피세포편평형태기본완정,내탄성막평정후도균균,손상명현감경.결론:TSM통과감소CEC수량、강저AngⅡ수평,체도보호대서실험성동맥죽양경화모형내피세포적작용.
AIM:To investigate the effect of TSM tablet on rat' s atherosclerotic model' s endothelial cell and explore the mechanism of it.METHODS:Fifty Wistar rats were randomly assigned into five groups:control group,model group,Simvastatin group,TSM high group,TSM low group.All groups were fed with high fat diet and vitamin D_3 except for control group to set up atherosclerosis model.After 12 weeks we detected circle endothelial cell,angiotensin Ⅱ and electron microscopy morphology of arteries.RESULTS:Level of circulating enthelia cell(CEC)and Ang Ⅱ in model rats were significantly higher.TSM can reduce level of the CEC and Ang Ⅱ.Model group rat' s artery endothelial cells were severely damaged under electron microscopy;rat's artery endothelial cells in TSM group were basically intact and its internal elastic membrane was unbroken on it thickness was even,without significant lesion.CONCLUSION:TSM by reducing the number of CEC,the level of Ang Ⅱ used for experimental atherosclerotic endothelial cell in rats has the protective effect.