CAJ | 학술논문

目的:探讨液基薄层细胞学检测(TCT)剩余细胞学标本进行p16和Ki-67的免疫细胞化学染色,作为宫颈细胞学检查未明确诊断意义的不典型鳞状上皮细胞(ASCUS)中的分流管理的应用价值.方法:对2008年11月至2009年7月在佛山市妇幼保健院就诊的98例细胞学检查结果为ASCUS的患者进行p16、Ki-67免疫细胞化学染色检测,并与阴道镜下病理活检结果比较.结果:p16、Ki-67表达阳性率随着宫颈病变程度的加重而升高,与病变级别呈正相关.p16在高级别鳞状上皮病变(HSIL)组和低级别鳞状上皮病变(LSIL)组中的阳性表达率与炎症组间比较,差异有统计学意义(P<0.05).Ki-157在HSIL组中的阳性表达率与LSIL组和炎症组比较,差异有统计学意义(P<0.05).p16和Ki-67的免疫细胞化学染色检出HSIL及鳞状细胞癌(SCC)的敏感度为83.3%(20/24)和91.7%(22/24);特异度为80.4%(74/92)和86.0%(74/86).结论:利用TCT剩余细胞学标本进行p16和Ki-67的免疫细胞化学染色,是一种有效、简便的ASCUS分流管理的生物学标记物,可提高HSIL的检出率,使高危患者得到及时有效的治疗.
목적:탐토액기박층세포학검측(TCT)잉여세포학표본진행p16화Ki-67적면역세포화학염색,작위궁경세포학검사미명학진단의의적불전형린상상피세포(ASCUS)중적분류관리적응용개치.방법:대2008년11월지2009년7월재불산시부유보건원취진적98례세포학검사결과위ASCUS적환자진행p16、Ki-67면역세포화학염색검측,병여음도경하병리활검결과비교.결과:p16、Ki-67표체양성솔수착궁경병변정도적가중이승고,여병변급별정정상관.p16재고급별린상상피병변(HSIL)조화저급별린상상피병변(LSIL)조중적양성표체솔여염증조간비교,차이유통계학의의(P<0.05).Ki-157재HSIL조중적양성표체솔여LSIL조화염증조비교,차이유통계학의의(P<0.05).p16화Ki-67적면역세포화학염색검출HSIL급린상세포암(SCC)적민감도위83.3%(20/24)화91.7%(22/24);특이도위80.4%(74/92)화86.0%(74/86).결론:이용TCT잉여세포학표본진행p16화Ki-67적면역세포화학염색,시일충유효、간편적ASCUS분류관리적생물학표기물,가제고HSIL적검출솔,사고위환자득도급시유효적치료.
Objective:To explore the relationship between p16, Ki-67 immunocytochemical stain and path-ological classification in cervival atypical squamous cells undefined significance (ASCUS) in residual cell of thinprep liquid-based cytology test (TCT).Methods :98 cases who suffering from ASCUS in TCT were col-lected in Foshan maternal and child health hospital from Nov, 2008 to Jul, 2009.And then the expressions of p16 and Ki-67 in the cervical cells were evaluated by immunocytochemical stain.The result was compared with the pathological result of colposcope blopsy.Results:The positive expression rate of p16, Ki-67 was raised accompanying with the severity of cervical disease, which was positively correlated with the degree of disease.There was statistical significant difference between the positive expression of p16 in HSIL and in-flammatory groups, which was similar to LSIL (P<0.05).And the positive expression of Ki-67 in HSIL was significant difference compared with other groups (P < 0.05).The sensitivity of p16 and Ki-67 was 83.3% (20/24) ,91.7% (22/24) and the specificity was 80.4% (74/92) , 86.0% (74/86) in immunochemistry stain.Conclusions :The detection of p16, Ki-67 via immunocytochemical stain in rasidual cells of TCT is an efficient and simple method to label the bio-marker in the administration of ASCUS, which can enhance the positive identification of HSIL and treat the high risk patient in time and effectively.