CAJ | 학술논문

为进一步了解海洋微藻对尿素的吸收和利用机制,揭示水体尿素浓度升高对近海浮游植物群落演替的潜在影响及对近海有害藻类水华(HABs)形成的促进作用.对海洋微藻脲酶活性的测定方法进行了研究.以典型赤潮藻东海原甲藻为实验材料.在Peers方法的基础上,探讨了失活处理时间、提取液pH值、酶促反应温度以及微藻生长阶段对脲酶活性的影响.结果表明,失活处理时间、提取液pH值、酶促反应温度以及微藻所处的生长阶段都对微藻的脲酶活性具有显著影响.海洋微藻脲酶活性的最佳测定条件为:100℃下失活处理时间>2min;提取液pH值7.9;反应温度应与微藻生长温度一致;指数生长初期的藻体具有较高的酶活,而平台期后酶活趋于稳定.
위진일보료해해양미조대뇨소적흡수화이용궤제,게시수체뇨소농도승고대근해부유식물군락연체적잠재영향급대근해유해조류수화(HABs)형성적촉진작용.대해양미조뇨매활성적측정방법진행료연구.이전형적조조동해원갑조위실험재료.재Peers방법적기출상,탐토료실활처리시간、제취액pH치、매촉반응온도이급미조생장계단대뇨매활성적영향.결과표명,실활처리시간、제취액pH치、매촉반응온도이급미조소처적생장계단도대미조적뇨매활성구유현저영향.해양미조뇨매활성적최가측정조건위:100℃하실활처리시간>2min;제취액pH치7.9;반응온도응여미조생장온도일치;지수생장초기적조체구유교고적매활,이평태기후매활추우은정.
Assay optimization of urease activity in marine microalgae was studied in laboratory for further understanding the uptake and utilization mechanism of marine microalgac for urea and revealing effect of the increase of urea concentration for community succession of phytoplankton and its potential contribution to the formation of HABs in coastal waters.Based on Peers'method,effect of inactivation time,pH value ofthe extract,incubation temperature and growth phase of microalgae on urease activity in a representative HABs species Prorocentrum donghaiense isolated from East China Sea were discussed.The results showed that inactivation time,pH value of the extract,incubation temperature and growth phase of microalgae all significantly influenced urease activity in microalgae.The optimum conditions for detecting urease activity in microalgae were:inactivation time>2min under 100℃,pH of extract 7.9,incubation temperature consistent with the growth temperature of the microalgue,urease activity in microalgae higher during early exponential phase and stabilized after stationary phase.