CAJ | 학술논문

目的了解肥胖型2型糖尿病OLETF鼠胰岛β细胞是否存在凋亡及β细胞凋亡与糖尿病发生发展的关系.方法 OLETF及对照鼠LETO各20只,胰腺切片用HE染色和TUNEL方法观察胰岛β细胞凋亡的情况.结果 (1)OLETF鼠糖尿病发病率为95%,LETO未发生糖尿病.(2)仅在OLETF鼠胰腺切片中观察到凋亡的β细胞数目增高的现象.(3)各周龄OLETF鼠胰岛β细胞凋亡的百分数明显高于LETO鼠(P＝1.26×10-17).(4)OLETF鼠胰岛β细胞凋亡的百分数在糖尿病发病后4周较糖尿病发病前增高.结论 OLETF鼠胰岛β细胞在糖尿病发病前存在高水平的凋亡,糖尿病发病后进一步增高.
목적료해비반형2형당뇨병OLETF서이도β세포시부존재조망급β세포조망여당뇨병발생발전적관계.방법 OLETF급대조서LETO각20지,이선절편용HE염색화TUNEL방법관찰이도β세포조망적정황.결과 (1)OLETF서당뇨병발병솔위95%,LETO미발생당뇨병.(2)부재OLETF서이선절편중관찰도조망적β세포수목증고적현상.(3)각주령OLETF서이도β세포조망적백분수명현고우LETO서(P＝1.26×10-17).(4)OLETF서이도β세포조망적백분수재당뇨병발병후4주교당뇨병발병전증고.결론 OLETF서이도β세포재당뇨병발병전존재고수평적조망,당뇨병발병후진일보증고.
Objective To determine whether the β cells apoptosis exits in the obesity associated type 2 diabetic rats OLETF and the relationship between the apoptosis and development of type 2 diabetes. Methods Twenty OLETFs and twenty LETOs as control were used. HE staining and TUNE were the way to examine apoptotic β cell in pancreatic sections. Results (1) The incidence of diabetes in OLETF rats was 95%. None of diabetic rat was found among LETO rats. (2) Apoptotic cells only appeared in pancreatic sections from OLETF rats. (3) The percentage of islet β cell apoptosis in OLETF rats was higher than that in LETO rats (P=1.26×10 -17 ). (4) In OLETF rats, the percentage of islet β cells apoptosis was higher in the ages of 4 weeks after the onset of diabetes than that in the ages of four weeks before the onset of diabetes. Conclusion There was a high level of β cell apoptosis in OLETF rats prior to the onset of diabetes and a further increase of apoptosis after diabetes.