中国寄生虫病防治杂志
中國寄生蟲病防治雜誌
중국기생충병방치잡지
CHINESE JOURNAL OF PARASITIC DISEASE CONTROL
2002年
3期
138-140
,共3页
弓形虫%IFN-γ%吲哚胺2,3过氧化酶(IDO)
弓形蟲%IFN-γ%吲哚胺2,3過氧化酶(IDO)
궁형충%IFN-γ%신타알2,3과양화매(IDO)
Toxoplasma gondii%IFN-γ%indoleamine2,3-dioxygenase(IDO)
目的探讨IFN-γ抑制人成纤维细胞内弓形虫增殖的机制. 方法用反相高效液相色谱法测吲哚胺2,3过氧化酶(IDO)活性;用3H-Uracil掺入法测弓形虫增殖;用反转录聚合酶链式反应测IDO mRNA的表达. 结果 IFN-γ能抑制人成纤维细胞内弓形虫增殖,培养上清液中检测到色氨酸被降解,生成了犬尿氨酸;培养液中加入足量色氨酸后,IFN-γ对弓形虫的抑制作用被部分逆转;用IDO特异引物扩增出一条662 bp DNA条带. 结论 IFN-γ抑制人成纤维细胞内弓形虫增殖是通过诱导细胞表达IDO活性降解色氨酸机制实现的.
目的探討IFN-γ抑製人成纖維細胞內弓形蟲增殖的機製. 方法用反相高效液相色譜法測吲哚胺2,3過氧化酶(IDO)活性;用3H-Uracil摻入法測弓形蟲增殖;用反轉錄聚閤酶鏈式反應測IDO mRNA的錶達. 結果 IFN-γ能抑製人成纖維細胞內弓形蟲增殖,培養上清液中檢測到色氨痠被降解,生成瞭犬尿氨痠;培養液中加入足量色氨痠後,IFN-γ對弓形蟲的抑製作用被部分逆轉;用IDO特異引物擴增齣一條662 bp DNA條帶. 結論 IFN-γ抑製人成纖維細胞內弓形蟲增殖是通過誘導細胞錶達IDO活性降解色氨痠機製實現的.
목적탐토IFN-γ억제인성섬유세포내궁형충증식적궤제. 방법용반상고효액상색보법측신타알2,3과양화매(IDO)활성;용3H-Uracil참입법측궁형충증식;용반전록취합매련식반응측IDO mRNA적표체. 결과 IFN-γ능억제인성섬유세포내궁형충증식,배양상청액중검측도색안산피강해,생성료견뇨안산;배양액중가입족량색안산후,IFN-γ대궁형충적억제작용피부분역전;용IDO특이인물확증출일조662 bp DNA조대. 결론 IFN-γ억제인성섬유세포내궁형충증식시통과유도세포표체IDO활성강해색안산궤제실현적.
Objective To explore the mechanism of IFN-γ to inhibit the proliferation of Toxoplasma gondii in human fibroblasts. Methods Indoleamine 2,3-dioxygenase(IDO) activity was determined by reverse-phase high performance liquid chromatography. The extent of the inhibition of the growth of T. gondii was measured by the incorporation of 3H-uracil. Expression of IDO mRNA was detected by RT-PCR. Results IFN-γ could induce human fibroblasts to express IDO activity and resist the replication of T. gondii. Depletion of tryptophan and accumulation of kynurenine in culture supernatant were found. A 662 bp DNA band standed for IDO mRNA was detected. Conclusion IFN-γ inhibits the proliferation of T. gondii in human fibroblasts by inducing IDO to degradate tryptophan.
