上海海洋大学学报
上海海洋大學學報
상해해양대학학보
JOURNAL OF SHANGHAI OCEAN UNIVERSITY
2009年
4期
421-427
,共7页
宓国强%练青平%王雨辰%沈土山
宓國彊%練青平%王雨辰%瀋土山
복국강%련청평%왕우신%침토산
翘嘴鳜%斑鳜%杂交子一代%胚胎发育
翹嘴鱖%斑鱖%雜交子一代%胚胎髮育
교취궐%반궐%잡교자일대%배태발육
Siniperca chuatsi%Siniperca scherzeri%hybrid F1%embryonic development
在ZOOM645S解剖镜下对翘嘴鳜(♀)×斑鳜(♂)人工杂交子一代胚胎发育各个阶段的发育时序和形态特征进行了观察.结果表明:受精卵为漂浮卵,微粘性,卵径1.6～1.8 mm.在水温(24±1)℃条件下,受精后1 h 45 min,胚盘隆起;2 h 30 min,进入卵裂期;3 h 15 min,进入桑椹期;4 h 15 min,进入囊胚期;11 h,进入原肠期;13 h 40 min,进入神经胚期;14 h 20 min ,进入胚孔封闭期;17 h 20 min,进入肌节出现期;20 h 10 min,进入视泡期;25 h,进入尾芽期;26 h 40 min,进入肌肉效应期;31 h,进入晶体出现期;35 h 10 min,进入心跳期;49 h 10 min,进入眼色素形成期;51 h 20 min,进入血液循环期;59 h 10 min后,开始出膜.整个胚胎发育时序和形态特征偏向于母本翘嘴鳜.
在ZOOM645S解剖鏡下對翹嘴鱖(♀)×斑鱖(♂)人工雜交子一代胚胎髮育各箇階段的髮育時序和形態特徵進行瞭觀察.結果錶明:受精卵為漂浮卵,微粘性,卵徑1.6～1.8 mm.在水溫(24±1)℃條件下,受精後1 h 45 min,胚盤隆起;2 h 30 min,進入卵裂期;3 h 15 min,進入桑椹期;4 h 15 min,進入囊胚期;11 h,進入原腸期;13 h 40 min,進入神經胚期;14 h 20 min ,進入胚孔封閉期;17 h 20 min,進入肌節齣現期;20 h 10 min,進入視泡期;25 h,進入尾芽期;26 h 40 min,進入肌肉效應期;31 h,進入晶體齣現期;35 h 10 min,進入心跳期;49 h 10 min,進入眼色素形成期;51 h 20 min,進入血液循環期;59 h 10 min後,開始齣膜.整箇胚胎髮育時序和形態特徵偏嚮于母本翹嘴鱖.
재ZOOM645S해부경하대교취궐(♀)×반궐(♂)인공잡교자일대배태발육각개계단적발육시서화형태특정진행료관찰.결과표명:수정란위표부란,미점성,란경1.6～1.8 mm.재수온(24±1)℃조건하,수정후1 h 45 min,배반륭기;2 h 30 min,진입란렬기;3 h 15 min,진입상심기;4 h 15 min,진입낭배기;11 h,진입원장기;13 h 40 min,진입신경배기;14 h 20 min ,진입배공봉폐기;17 h 20 min,진입기절출현기;20 h 10 min,진입시포기;25 h,진입미아기;26 h 40 min,진입기육효응기;31 h,진입정체출현기;35 h 10 min,진입심도기;49 h 10 min,진입안색소형성기;51 h 20 min,진입혈액순배기;59 h 10 min후,개시출막.정개배태발육시서화형태특정편향우모본교취궐.
Fertilized eggs were obtained by the crossing of Siniperca chuatsi (♀) × Siniperca scherzeri (♂) with artificial manipulation, and the embryonic development process and the morphological characters at various stages were recorded with a ZOOM645S microscope. Results showed that the fertilized egg was floating and slightly sticky with a diameter of 1.6-1.8 mm. Under the condition of （24±1）℃water temperature, 1 hour and 45 minutes after fertilizing, the fertilized egg developed to the formation of blastoderm, 2 hours and 30 minutes to cleavage stage, 3 hours to morula stage, 4 hours and 15 minutes to blastula stage, 11 hours to gastrula stage, 13 hours and 40 minutes to neurula stage, 14 hours and 20 minutes to blastopore closing stage, 17 hours and 20 minutes to myocoma formation stage, 20 hours and 10 minutes to optic vesicle stage, 25 hours to tail- bud stage, 26 hours and 40 minutes to muscular effect stage, 31 hours to eye lens formed stage, 35 hours and 10 minutes to heart working stage, 49 hours and 10 minutes eye pigmenting stage, 51 hours and 20 minutes to blood circulation stage, and 59 hours and 10 minutes, fry began to be hatched out. The overall embryonic development process and the morphological characters were more similar to those of its maternal fish.
