CAJ | 학술논문

合成了带有活性末端的寡聚N-异丙基丙烯酰胺（ONIPAAm）, 并考察了其温度敏感性质. 以ONIPAAm作为免疫分析的载体与鼠IgG偶联, 以四磺基铁酞菁为标记物, 以羊抗鼠IgG抗体为分析模型, 建立了竞争型热敏相分离荧光免疫分析新系统. 羊抗鼠IgG抗体在0～1 500 ng/mL范围内与体系相对荧光强度呈良好的线性关系, 检测限为2 ng/mL.
합성료대유활성말단적과취N-이병기병희선알（ONIPAAm）, 병고찰료기온도민감성질. 이ONIPAAm작위면역분석적재체여서IgG우련, 이사광기철태정위표기물, 이양항서IgG항체위분석모형, 건립료경쟁형열민상분리형광면역분석신계통. 양항서IgG항체재0～1 500 ng/mL범위내여체계상대형광강도정량호적선성관계, 검측한위2 ng/mL.
A temperature-sensitive polymer containing a terminal carboxyl group was prepared by copolymerization of N-isopropylacrylamide with mercaptoacetic acid. This polymer was conjugated to the IgG with EDCI and used to develop a novel polymer-mimetic enzyme competitive immunoassay method for determination of anti-IgG with iron-tetrasulfonatophthalocyanine as a labelling reagent to catalyze the reaction of p-hydroxyphenyl acetic acid (HPA) and hydrogen peroxide in alkaline medium. The calibration graph for anti-IgG was linear over the range of 01 500 ng/mL with a detection limit of 2.0 ng/mL.