CAJ | 학술논문

目的：探讨鼻咽癌负相关基因 BRD7对鼻咽癌细胞系 HNE1生长的影响。方法：构建 BRD7基因真核表达载体 pcDNA3.1(＋ )/BRD7重组体，采用脂质体介导转染技术，将 BRD7真核表达重组质粒和空载体质粒分别导入鼻咽癌细胞系 HNE1， Southern杂交和 RT PCR分别检测外源性 DNA的整合和 BRD7基因的表达，并借助细胞生长曲线、软琼脂集落形成试验、流式细胞计数和裸鼠接种方法对转染细胞的生物学行为进行了检测。结果：转染 BRD7基因的 HNE1生长倍增时间为 53 h，较 HNE1（ 23.9 h）和空载体转染 HNE1（ 24.1 h）明显延长，流式细胞仪表明， BRD7表达升高延缓细胞由 G0～ G1期进入 S期， BRD7转染 HNE1在软琼脂中集落形成率较对照组显著下降（ P< 0.01），裸鼠接种试验显示 BRD7基因转染细胞 HNE1生长速度受到抑制。结论： BRD7基因重表达有助于 HNE1的恶性表型的逆转； BRD7是一个鼻咽癌相关的抑瘤基因良好的候选者。
목적：탐토비인암부상관기인 BRD7대비인암세포계 HNE1생장적영향。방법：구건 BRD7기인진핵표체재체 pcDNA3.1(＋ )/BRD7중조체，채용지질체개도전염기술，장 BRD7진핵표체중조질립화공재체질립분별도입비인암세포계 HNE1， Southern잡교화 RT PCR분별검측외원성 DNA적정합화 BRD7기인적표체，병차조세포생장곡선、연경지집락형성시험、류식세포계수화라서접충방법대전염세포적생물학행위진행료검측。결과：전염 BRD7기인적 HNE1생장배증시간위 53 h，교 HNE1（ 23.9 h）화공재체전염 HNE1（ 24.1 h）명현연장，류식세포의표명， BRD7표체승고연완세포유 G0～ G1기진입 S기， BRD7전염 HNE1재연경지중집락형성솔교대조조현저하강（ P< 0.01），라서접충시험현시 BRD7기인전염세포 HNE1생장속도수도억제。결론： BRD7기인중표체유조우 HNE1적악성표형적역전； BRD7시일개비인암상관적억류기인량호적후선자。
Objective：This study was designed to explore the effect of BRD7 gene negative associated with nasopharyngeal carcinoma (NPC) on the growth of NPC cell line HNE1. Methods： The mammal expression vector of BRD7, pcDNA3.1(＋ )/BRD7, was constructed and transfected into HNE1 cell. Stable G418 resistant clones were isolated, and the integration of the exogenous vector DNA and the expression of BRD7 gene were detected by Southern blot and RT PCR respectively. Finally the cytobiological characterization of positive clone (B 4) was analyzed by using population double time (PDT), soft agar assay, cytometry, and xenograft. Results： The PDT of G418 resistant HNE1 cell with epression of BRD7 was 53 h and significantly longer than that of vector transfected HNE1 cell and untransfected HNE1 (P< 0.01). Flow cytometric data shown that more BRD7 transfected cells went into phase G0－ G1 than controls. And it also presented decreased clonogenicity and tomorigenicity in soft agar assay and tumor formation in nude mice. Conclusion： The reexpression of BRD7 could favor the malignant phenotype revision of NPC cells. And BRD7 gene might be a good candidate of tumor suppressor gene correlated with NPC.