CAJ | 학술논문

目的:探讨羟基磷灰石/磷酸三钙(HA/TCP)复合材料作为组织工程载体的可能;为基质材料表面改性提供实验基础.方法:应用人骨髓基质细胞(hBMSCs)和脐静脉内皮细胞(UVECs)和HA/TCP进行混合培养,应用光学显微镜、荧光显微镜及扫描电子显微镜对其进行观察.结果:人骨髓基质细胞和脐静脉内皮细胞在HA/TCP表面均生长良好,并可观察到细胞长入基质材料微孔内的改变.结论:羟基磷灰石/磷酸三钙(HA/TCP)复合材料可作为细胞移植的载体.
목적:탐토간기린회석/린산삼개(HA/TCP)복합재료작위조직공정재체적가능;위기질재료표면개성제공실험기출.방법:응용인골수기질세포(hBMSCs)화제정맥내피세포(UVECs)화HA/TCP진행혼합배양,응용광학현미경、형광현미경급소묘전자현미경대기진행관찰.결과:인골수기질세포화제정맥내피세포재HA/TCP표면균생장량호,병가관찰도세포장입기질재료미공내적개변.결론:간기린회석/린산삼개(HA/TCP)복합재료가작위세포이식적재체.
Objective To explore the possibility of hydroxyapatite/tricalcium phosphate(HA/TCP)matrix scaffold as cell carriers in tissue engineering,and to provide a direct evidence for the resurfacing of matrix scaffold. Methods Human bone marrow stromal cells (hBMSCs) and umbilical vein endothelial cells(UVECs) were co-cultured on the surface of matrix scaffold.The morphologic characters during culture on HA/TCP matrix scaffold′s surfaces were checked by light microscope,fluorescence microscope,and scanning electronic microscope.Results Both human bone marrow stromal cells and human umbilical vein endothelial cells showed good biocompatibility with HA/TCP matrix scaffold.The cell growth into the micropore of HA/TCP matrix scaffold could be seen under both fluorescence microscopy and scaning electron microscopy.Conclusion HA/TCP matrix scaffold can be used as a vehicle for the cell transplantation.