CAJ | 학술논문

采用高效液相色谱-紫外-荧光和高效液相色谱-紫外-质谱联用法,证明胃癌患者胃液中存在的荧光物质主要为色氨酸.建立了反相高效液相色谱-双波长同时紫外检测法定量分析胃液中色氨酸和利多卡因的方法.使用Kromasil C_(18)柱(150 mm×4.6 mm, 5 μm),以含0.1%三氟乙酸的甲醇溶液与含0.1%三氟乙酸水溶液为流动相,梯度洗脱分析.色氨酸使用278 nm检测,浓度在0.5～200 mg/L范围内峰面积对浓度的线性相关系数为0.9994;利多卡因使用254 nm检测,浓度在20～5000 mg/L范围内相关系数为0.9992.色氨酸和利多卡因的检出限分别为0.15和5 mg/L;平均加标回收率分别为70.8%～110.4%(色氨酸) 和87.1%～116.2% (利多卡因).利用本方法对38例胃癌患者和48例非胃癌患者的胃液进行测试,两组胃液中色氨酸的含量存在一定的差异.
채용고효액상색보-자외-형광화고효액상색보-자외-질보련용법,증명위암환자위액중존재적형광물질주요위색안산.건립료반상고효액상색보-쌍파장동시자외검측법정량분석위액중색안산화리다잡인적방법.사용Kromasil C_(18)주(150 mm×4.6 mm, 5 μm),이함0.1%삼불을산적갑순용액여함0.1%삼불을산수용액위류동상,제도세탈분석.색안산사용278 nm검측,농도재0.5～200 mg/L범위내봉면적대농도적선성상관계수위0.9994;리다잡인사용254 nm검측,농도재20～5000 mg/L범위내상관계수위0.9992.색안산화리다잡인적검출한분별위0.15화5 mg/L;평균가표회수솔분별위70.8%～110.4%(색안산) 화87.1%～116.2% (리다잡인).이용본방법대38례위암환자화48례비위암환자적위액진행측시,량조위액중색안산적함량존재일정적차이.
The HPLC-UV-Fluorescence technique and HPLC-UV-MS technique were used to determine fluorescence molecule in gastric juice of patients with malignant gastric cancer,which is identified as tryptophan. Lidocaine was detected in gastric juice of patients who were examined by gastric endoscopy. A method was developed to determine concentration of tryptophan and lidocaine in gastric juice using HPLC-UV technique,in which Kromasil C_(18) column(150 mm×4.6 mm,5μm) and methanol(containing 0.1% trifluoroacetic acid (TFA))-water(containing 0. 1% TFA) as gradient mobile phase were used. The linear relationships were obtained between the concentration of tryptophan in the range of 0.5 - 200 mg/L and its peak area at 278 run with correlation coefficient of 0.9994,and between the concentration of lidocaine in the range of 20 -5000 mg/L and its peak area at 254 nm with correlation coefficient of 0.9992. The limit of detection is 0.15 mg/L for tryptophan and 5 mg/L for lidocaine. The recovery is 70. 8% - 110. 4% for tryptophan and 87. 1% -116.2% for lidocaine. 38 gastric juice samples of patients with malignant gastric disease and 48 gastric juice samples of patients with benign gastric disease were tested and the concentration of tryptophan in those two groups of gastric juice samples was apparently different.