细胞与分子免疫学杂志
細胞與分子免疫學雜誌
세포여분자면역학잡지
2009年
10期
903-906
,共4页
龚卫娟%王海洋%范敏其%龚春香%刘丹%季明春
龔衛娟%王海洋%範敏其%龔春香%劉丹%季明春
공위연%왕해양%범민기%공춘향%류단%계명춘
MHCⅠ类相关蛋白A%NK细胞%细胞毒活性%增殖%凋亡
MHCⅠ類相關蛋白A%NK細胞%細胞毒活性%增殖%凋亡
MHCⅠ류상관단백A%NK세포%세포독활성%증식%조망
MICA%NK cell%cytotoxicity%proliferation%apoptosis
目的:研究体外重组可溶性MHC Ⅰ类相关蛋白A(sMICA)对NK细胞杀伤靶细胞活性、分泌IFN-γ、增殖和凋亡的影响.方法:将重组sMICA蛋白与人外周血NK细胞相互作用过夜后,流式细胞仪检测NK细胞杀伤K562靶细胞的能力;ELISA检测培养上清IFN-γ浓度;MTS/PMS法检测sMICA对NK细胞增殖的影响;给NK细胞标记Annexin V和碘化丙啶检测凋亡情况.结果:可溶性MICA抑制NK细胞杀伤K562细胞的活性,下调IFN-γ的分泌,却对NK细胞的增殖和凋亡没有影响.结论:肿瘤细胞表面脱落的sMICA抗原可通过抑制NK细胞活性而逃逸机体的免疫监视功能.
目的:研究體外重組可溶性MHC Ⅰ類相關蛋白A(sMICA)對NK細胞殺傷靶細胞活性、分泌IFN-γ、增殖和凋亡的影響.方法:將重組sMICA蛋白與人外週血NK細胞相互作用過夜後,流式細胞儀檢測NK細胞殺傷K562靶細胞的能力;ELISA檢測培養上清IFN-γ濃度;MTS/PMS法檢測sMICA對NK細胞增殖的影響;給NK細胞標記Annexin V和碘化丙啶檢測凋亡情況.結果:可溶性MICA抑製NK細胞殺傷K562細胞的活性,下調IFN-γ的分泌,卻對NK細胞的增殖和凋亡沒有影響.結論:腫瘤細胞錶麵脫落的sMICA抗原可通過抑製NK細胞活性而逃逸機體的免疫鑑視功能.
목적:연구체외중조가용성MHC Ⅰ류상관단백A(sMICA)대NK세포살상파세포활성、분비IFN-γ、증식화조망적영향.방법:장중조sMICA단백여인외주혈NK세포상호작용과야후,류식세포의검측NK세포살상K562파세포적능력;ELISA검측배양상청IFN-γ농도;MTS/PMS법검측sMICA대NK세포증식적영향;급NK세포표기Annexin V화전화병정검측조망정황.결과:가용성MICA억제NK세포살상K562세포적활성,하조IFN-γ적분비,각대NK세포적증식화조망몰유영향.결론:종류세포표면탈락적sMICA항원가통과억제NK세포활성이도일궤체적면역감시공능.
AIM: To study the effects of recombinant soluble MHC class Ⅰ chain-related protein A (sMICA) on the cytotoxicity, secretion of IFN-γ, proliferation and apoptosis of peripheral NK cells. METHODS: After NK cells were cocultured with recombinant soluble MICA proteins overnight, the cytotoxicity of NK cell on target cells was detected by flow cytometry. The supemant was collected to determine the concentration of IFN-γ by ELISA. The proliferation of NK cells to sMICA was detected by MTS/PMS. NK cells were labeled with annexin V and PI to analyze their apoptosis. RESULTS: Soluble MICA inhibited the cytotoxicity of NK cells and down-regulated the secretion of IFN-γ, but it showed no effects on the proliferation and apoptosis of freshly isolated peripheral NK cells. CONCLUSION: The soluble MICA shedding from tumor cells could be a pathway of cancer immune evasion by down-regulating the biologic activities of NK cells.