CAJ | 학술논문

建立了基质固相分散萃取(matrix solid phase dispersion, MSPD)-反相高效液相色谱(reversed phase high performance liquid chromatography, RP-HPLC)检测利谷隆、灭草隆、敌草隆和绿麦隆4种苯脲类除草剂的新方法.茶叶样品与C18填料混合碾磨后与中性氧化铝一起装柱,二氯甲烷为淋洗剂,使样品的萃取与净化一步完成.讨论了MSPD法和色谱分析的各种影响因素,最佳实验条件下灭草隆在0.01～10.00 mg/L范围内线性关系良好,检测限为5 μg/L;绿麦隆、敌草隆和利谷隆在0.025～10.00 mg/L范围内线性关系良好,检测限为10 μg/L.4种除草剂在茶叶样品中的回收率在83.5%～100.4%之间,相对标准偏差小于7.1%.该方法简单、快速、干扰少、回收率高,符合茶叶中除草剂残留分析要求.
건립료기질고상분산췌취(matrix solid phase dispersion, MSPD)-반상고효액상색보(reversed phase high performance liquid chromatography, RP-HPLC)검측리곡륭、멸초륭、활초륭화록맥륭4충분뇨류제초제적신방법.다협양품여C18전료혼합년마후여중성양화려일기장주,이록갑완위림세제,사양품적췌취여정화일보완성.토론료MSPD법화색보분석적각충영향인소,최가실험조건하멸초륭재0.01～10.00 mg/L범위내선성관계량호,검측한위5 μg/L;록맥륭、활초륭화리곡륭재0.025～10.00 mg/L범위내선성관계량호,검측한위10 μg/L.4충제초제재다협양품중적회수솔재83.5%～100.4%지간,상대표준편차소우7.1%.해방법간단、쾌속、간우소、회수솔고,부합다협중제초제잔류분석요구.
A new method for the simultaneous determination of 4 herbicides (Monuron, Chlortoluron, Diuron and Linuron) was established with MSPD-RP-HPLC. The mixture of tea samples and C18 stuffing were pulverized, then mixed with alumina packed to a syringe column, the extraction and purification of the samples were carried out with one-step using dichloromethane as eluting reagent. The effecting factors of MSPD and conditions of the chromatography were discussed. Under optimum conditions, the linear ranges of Monuron was 0.01～10.0 mg/L with 5 μg/L of the detection limits, and the linear ranges of Chlortoluron, Diuron and Linuron were 0.025～10.0 mg/L with 10 μg/L of the detection limits. The average recoveries of 4 herbicides ranged from 83.5% to100.4% with less than 7.1% of the relative standard deviations in tea samples. The method suggested was simple, rapid, and less interference with high recovery rate, and meet the analytical requirements of herbicide residues in tea.