CAJ | 학술논문

目的建立高脂血症实验动物模型新型方法.方法 30只小鼠随机分为空白对照组、阳性对照组及模型组.空白对照组喂饲标准饲料,阳性对照组喂饲高脂饲料;模型组饲以标准饲料和牛奶,30 d后检测血清和肝脏胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)及肝脏指数(LI),光镜观察肝脏病理变化.结果与空白组相比,模型组LI及血液和血清中TC、TG、LDL-C明显升高,而HDL-C明显降低.光镜下模型组肝脏均出现弥漫性肝细胞脂肪变性和炎症病灶.结论小鼠喂饲30 d标准饲料和牛奶,可建立理想的高脂血症模型.
목적 건립고지혈증실험동물모형신형방법.방법 30지소서수궤분위공백대조조、양성대조조급모형조.공백대조조위사표준사료,양성대조조위사고지사료;모형조사이표준사료화우내,30 d후검측혈청화간장담고순(TC)、감유삼지(TG)、저밀도지단백담고순(LDL-C)、고밀도지단백담고순(HDL-C)급간장지수(LI),광경관찰간장병리변화.결과 여공백조상비,모형조LI급혈액화혈청중TC、TG、LDL-C명현승고,이HDL-C명현강저.광경하모형조간장균출현미만성간세포지방변성화염증병조.결론 소서위사30 d표준사료화우내,가건립이상적고지혈증모형.
Aim To establish an ideal hyperlipidemia animal model by a new way.Methods 30 mice were randomly divided into control group, positive control group and model group.The mice of control group were only fed with a standard diet.Those of positive control group were fed with high fat diet.Those of model group were fed with a standard diet and enough milk.After 30 days the TG, TC, HDL-C and LDL-C of serum and hepatic and LI were detected and pathological changes in the liver of mice were observed microscopically.Results Compared with the control group, the mice of model group developed hyperlipidemia with LI and the serum and the hepatic TG, TC, LDL-C elevated significantly, while HDL-C were significantly lower.The histopathological research showed hepatocellular macrovesicular steatosis and hepatitis in the model group.Conclusion An ideal model of hyperlipidemia is successfully established with standard diet and milk fed to mice for 30 days.