CAJ | 학술논문

目的:探讨柴胡疏肝散及其拆方的抗抑郁作用及机制.方法:将SD大鼠随机分为正常对照组、模型对照组、全方干预组、拆方干预I组和II组、氟西汀对照组.采用慢性轻度不可预见性应激配合孤养复制抑郁模型;运用敞箱试验和蔗糖水消耗实验观察大鼠行为改变;荧光实时定量PCR(FQ-PCR)检测各组大鼠海马组织细胞外调节蛋白激酶(extracellular regulated protein kinases 1/2, ERK1/2) mRNA表达水平. 结果:与正常对照组比较,模型组大鼠海马组织ERK1/2 2-ΔΔCT 显著降低,差异具有统计学意义(P<0.01),表明模型组海马ERK1/2 mRNA表达明显下调.与模型组比较,全方组、氟西汀组大鼠海马组织ERK1/2 2-ΔΔCT均明显升高,差异具有统计学意义(P<0.05或P<0.01);拆方I组ERK1 2-ΔΔCT亦明显上调,差异具有统计学意义(P<0.05);而拆方II组海马组织ERK1/2 2-ΔΔCT均无明显增高,与模型组比较差异无统计学意义(P>0.05).结论:慢性轻度不可预见性刺激结合孤养可诱导大鼠脑组织ERK1/2 mRNA 表达下调,柴胡疏肝散可能通过促进海马组织ERK1/2 mRNA的表达,改善抑郁大鼠模型的行为学异常,发挥抗抑郁作用;拆方I组也具有较好的抗抑郁作用,但作用不及全方组;拆方II组则无明显抗抑郁作用,但具增强抗抑郁作用.
목적:탐토시호소간산급기탁방적항억욱작용급궤제.방법:장SD대서수궤분위정상대조조、모형대조조、전방간예조、탁방간예I조화II조、불서정대조조.채용만성경도불가예견성응격배합고양복제억욱모형;운용창상시험화자당수소모실험관찰대서행위개변;형광실시정량PCR(FQ-PCR)검측각조대서해마조직세포외조절단백격매(extracellular regulated protein kinases 1/2, ERK1/2) mRNA표체수평. 결과:여정상대조조비교,모형조대서해마조직ERK1/2 2-ΔΔCT 현저강저,차이구유통계학의의(P<0.01),표명모형조해마ERK1/2 mRNA표체명현하조.여모형조비교,전방조、불서정조대서해마조직ERK1/2 2-ΔΔCT균명현승고,차이구유통계학의의(P<0.05혹P<0.01);탁방I조ERK1 2-ΔΔCT역명현상조,차이구유통계학의의(P<0.05);이탁방II조해마조직ERK1/2 2-ΔΔCT균무명현증고,여모형조비교차이무통계학의의(P>0.05).결론:만성경도불가예견성자격결합고양가유도대서뇌조직ERK1/2 mRNA 표체하조,시호소간산가능통과촉진해마조직ERK1/2 mRNA적표체,개선억욱대서모형적행위학이상,발휘항억욱작용;탁방I조야구유교호적항억욱작용,단작용불급전방조;탁방II조칙무명현항억욱작용,단구증강항억욱작용.
Objective To explore the antidepressant effect and mechanism of Chaihu Shugan San (CHSGS) composition, a compound traditional Chinese herb medicine and its components. Methods Rats were randomly divided into 6 groups: normal control group, model control group, a CHSGS group, a component I group, a component II group and a fluoxetine control group. The depression model was replicated by chronic unpredictable mild stress and single house for 28 d. Behavioral scores of the rats were detected by Open-field test and sucrose solution consumption test, and ERK1/2 mRNA expression in the hippocampus tissue was assayed by real-time fluorescent quantitative PCR. Results ERK1/2 mRNA expression was down-regulated in the depression model group compared with the normal control group (P<0.01). Compared with the model group, ERK1/2 mRNA expression in the CHSGS group and fluoxetine group was both up-regulated (P<0.05 or P<0.01); and only ERK1 mRNA expression in the component I group was up-regulated (P<0.05). No significant difference existed between the component II group and the model group (P>0.05). Conclusion Isolated-living condition and chronic mild unpredictable stress can down-regulate the expression of ERK1/2 mRNA in the hippocampus tissue. CHSGS may exert an antidepressant effect through increasing the expression of ERK1/2 mRNA in the hippocampus, component I may play an important role in its antidepression effect, while compatibility of the use of component II can enhance the antidepressant efficacy.