CAJ | 학술논문

目的探讨阿托伐他汀对于脓毒症大鼠模型肿瘤坏死因子α的影响.方法选择由大连医科大学实验动物中心提供的体质量184～202 g健康雄性SD (Sprague-Dawley)大鼠,采用盲肠结扎法(CLP)制作大鼠脓毒症模型,随机分为对照组、脓毒症组、阿托伐他汀低剂量组和阿托伐他汀高剂量组,各组分别在术后0,3,6,12,24h各取5只大鼠采血,分离血浆检测TNF-α的浓度,观察和比较各组大鼠活动度、小肠组织病理改变.采用SPSS 13.0软件包,于各时间点行重复测量资料方差分析.结果 0h各组血浆TNF-α浓度比较差异无统计学意义(P＞0.05).对照组血浆TNF-α浓度变化不明显,在3,6,12,24h阿托伐他汀低剂量组与阿托伐他汀高剂量组较脓毒症组和对照组、比较差异均具有统计学意义(P＜0.01),明显低于脓毒症组.阿托伐他汀高剂量组较阿托伐他汀低剂量组血浆TNF-α浓度明显降低(P＜0.01),差异具有统计学意义.脓毒症组大鼠脓毒症表现最强,阿托伐他汀组明显减轻,高剂量组较低剂量组减轻更为明显,对照组没有相关表现或仅有轻微表现.结论阿托伐他汀可以抑制TNF-α的表达,减轻炎症反应.
목적 탐토아탁벌타정대우농독증대서모형종류배사인자α적영향.방법 선택유대련의과대학실험동물중심제공적체질량184～202 g건강웅성SD (Sprague-Dawley)대서,채용맹장결찰법(CLP)제작대서농독증모형,수궤분위대조조、농독증조、아탁벌타정저제량조화아탁벌타정고제량조,각조분별재술후0,3,6,12,24h각취5지대서채혈,분리혈장검측TNF-α적농도,관찰화비교각조대서활동도、소장조직병리개변.채용SPSS 13.0연건포,우각시간점행중복측량자료방차분석.결과 0h각조혈장TNF-α농도비교차이무통계학의의(P＞0.05).대조조혈장TNF-α농도변화불명현,재3,6,12,24h아탁벌타정저제량조여아탁벌타정고제량조교농독증조화대조조、비교차이균구유통계학의의(P＜0.01),명현저우농독증조.아탁벌타정고제량조교아탁벌타정저제량조혈장TNF-α농도명현강저(P＜0.01),차이구유통계학의의.농독증조대서농독증표현최강,아탁벌타정조명현감경,고제량조교저제량조감경경위명현,대조조몰유상관표현혹부유경미표현.결론 아탁벌타정가이억제TNF-α적표체,감경염증반응.
Objective To investigate the influence of atorvastatin on tumor necrosis factor - α (TNF- α) of sepsis rats.Methods Sepsis models were established using male SD rats by cecal ligation and puncture (CLP).A total of 100 healthy rats were divided into 4 groups ( n =25) randomly ( random number):sham- operation group,CLP group,low- dose atorvastatin group and high -dose Atorvastatin group.Blood samples of 5 rats in each group were collected at postoperative 0,3,6,12 and 24 hours,to detect tumor necrosis factor - α (TNF - α) levels in plasma.Observe and compare the mobility of rats in each group and specimens of small intestine were taken for histopathological examination by optical microscope.Results At 0 hour,plasmic TNF - α levels in 4 groups were statistically equal (P ＞ 0.05 ).In plasma of sham - operation group,changes of TNF - α levels were not obvious.Compared with CLP group,TNF - α levels in low - dose and high - dose Atorvastatin groups were both significantly lower ( P ＜0.01 ) at postoperative 3,6,12 and 24 hours.And TNF-α levels in high -dose Atorvastatin group were significantly lower than those in low - dose group ( P ＜ 0.01 ) at the 4 time points.The mortality of sepsis was higher in CLP model group than other groups,That of Atorvastatin group was significantly lower than CLP model group but higher than control group and high - dose group was lower than low - dose group.Conclusions Atorvastatin can inhibit the expression of TNF - α in blood plasma of sepsis rats and reduce inflammatory reaction.