CAJ | 학술논문

目的探讨促凋亡信号和抗凋亡信号在特发性肺纤维化(idiopathic pulmonary fibrosis,IPF)发生中的意义.方法应用末端原位杂交和免疫组织化学技术,检测12例IPF患者的肺活检组织和10例正常对照肺组织肺泡和支气管上皮细胞凋亡情况及P53、P21、Bax和Bcl-2蛋白表达的变化.结果 IPF患者肺组织的肺泡和支气管上皮细胞凋亡率明显上调(U=0.0,P＜0.01),肺泡和支气管上皮细胞中P53、P21、Bax蛋白表达上调(U=5.0～35.5,P值均＜0.05),肺泡和支气管上皮细胞中Bcl-2蛋白表达下调(U值分别为12.0、18.0,P＜0.01).相关分析结果表明,肺泡和支气管上皮细胞P53、P21、Bax蛋白表达与其凋亡率之间均呈显著正相关(r值为0.436～0.806,P值均＜0.05),肺泡和支气管上皮细胞Bcl-2蛋白表达与其凋亡率之间均呈显著负相关(r值为-0.715～-0.699,P值均＜0.01).结论肺纤维化时肺泡和支气管上皮细胞凋亡率增高,P53、P21、Bax蛋白表达增强,Bcl-2蛋白表达减弱,这些因素在肺纤维化的发生和发展中起重要作用.
목적 탐토촉조망신호화항조망신호재특발성폐섬유화(idiopathic pulmonary fibrosis,IPF)발생중적의의.방법 응용말단원위잡교화면역조직화학기술,검측12례IPF환자적폐활검조직화10례정상대조폐조직폐포화지기관상피세포조망정황급P53、P21、Bax화Bcl-2단백표체적변화.결과 IPF환자폐조직적폐포화지기관상피세포조망솔명현상조(U=0.0,P＜0.01),폐포화지기관상피세포중P53、P21、Bax단백표체상조(U=5.0～35.5,P치균＜0.05),폐포화지기관상피세포중Bcl-2단백표체하조(U치분별위12.0、18.0,P＜0.01).상관분석결과표명,폐포화지기관상피세포P53、P21、Bax단백표체여기조망솔지간균정현저정상관(r치위0.436～0.806,P치균＜0.05),폐포화지기관상피세포Bcl-2단백표체여기조망솔지간균정현저부상관(r치위-0.715～-0.699,P치균＜0.01).결론 폐섬유화시폐포화지기관상피세포조망솔증고,P53、P21、Bax단백표체증강,Bcl-2단백표체감약,저사인소재폐섬유화적발생화발전중기중요작용.
Objective To investigate the changes and significance of cell apoptosis,P53,P21,Bax and Bcl-2 protein in epithelial cells from patients with idiopathic pulmonary fibrosis (IPF). Methods Cell apoptosis and the expressions of P53,P21,Bax and Bcl-2 protein in lung tissues from 12 patients with IPF (IPF group) and 10 normal controls (control group) were detected by TUNEL(terminal deoxynucleotide transferase-mediated deoxyuridine triphosphate-biotin nick end-labeling) and immunohistochemistry. Results Compared with that of control group,the percentage of apoptosis in alveolar epithelial cells and bronchial cells of IPF group was higher (all U = 0.0, all P＜0.01). The expressions of P53, P21, Bax protein in alveolar epithelial cells of IPF group were higher than those of control group( U = 5.0,25.5,27.0, all P＜0.05); and the expressions in bronchial cells of IPF group were also higher than those of control group( U =5.0,35.5,25.0,all P＜0.01). The expressions of Bcl-2 protein in alveolar epithelial cells and bronchial cells of IPF group were higher than those of control group( U = 12.0,18.0, all P＜0.01 ). There was a significant correlation between the percentage of apoptosis and P53, P21, Bax protein expression ( rs =0. 436-0. 806 ,all P＜0.01). The correlation of the percentage of apoptosis and Bcl-2 protein expression was also significant (rs=0. 715--0. 699,all P＜0.01). Conclusions The apoptosis percentage of epithelial cells and the expression of P53, P21, Bax protein are up-regulated in lung tissues of IPF, the expression of Bcl-2 protein is down-regulated,which may play an important role in the development of the disease.