CAJ | 학술논문

目的了解宫颈上皮内瘤变(CIN)宫颈癌患者下生殖道解脲脲原体感染情况,初步探讨解脲脲原体(UU)、HPV与CIN/宫颈癌的相关性.方法选取2005年9月至2009年2月中山大学附属第六医院及第二医院因宫颈上皮内瘤变或宫颈鳞癌住院的患者124例为病例组.随即抽取同期医院体检中心健康体检女性300例为对照组.采用荧光定量PCR方法进行宫颈分泌物解脲脲原体DNA检测.用杂交捕获Ⅱ代和导流杂交基因芯片技术检测HPV感染情况.结果病例组解脲脲原体阳性率68.5%,对照组解脲脲原体阳性率38.7%.病例组和对照组之间UU阳性率比较,差异有统计学意义.124例行HPV检测病例中,UU和HPV均阳性者占58.9%.结论女性下生殖道解脲脲原体感染与宫颈上皮内瘤变/宫颈癌有一定的相关性.解脲脲原体感染与宫颈病变程度相关,尤其与高度宫颈上皮内病变和宫颈癌相关.UU对HPV感染导致的CIN/宫颈癌可能存在协同作用.
목적 료해궁경상피내류변(CIN)궁경암환자하생식도해뇨뇨원체감염정황,초보탐토해뇨뇨원체(UU)、HPV여CIN/궁경암적상관성.방법 선취2005년9월지2009년2월중산대학부속제륙의원급제이의원인궁경상피내류변혹궁경린암주원적환자124례위병례조.수즉추취동기의원체검중심건강체검녀성300례위대조조.채용형광정량PCR방법진행궁경분비물해뇨뇨원체DNA검측.용잡교포획Ⅱ대화도류잡교기인심편기술검측HPV감염정황.결과 병례조해뇨뇨원체양성솔68.5%,대조조해뇨뇨원체양성솔38.7%.병례조화대조조지간UU양성솔비교,차이유통계학의의.124례행HPV검측병례중,UU화HPV균양성자점58.9%.결론 녀성하생식도해뇨뇨원체감염여궁경상피내류변/궁경암유일정적상관성.해뇨뇨원체감염여궁경병변정도상관,우기여고도궁경상피내병변화궁경암상관.UU대HPV감염도치적CIN/궁경암가능존재협동작용.
Objective To explore the relationship of infection of Ureaplasma urealyticum(UU),HPV loading dose and CIN or cervical cancer, also the relationship of infection of Ureaplasma urealyticum,HPV genotype and CIN or cervical cancer. Methods One hundred and twenty-four patients in The sixth and second Affiliated Hospital of Sun Yat-sen University with CIN or cervical cancer were selected to be case group from September 2005 to January 2009. Three hundred health examining women were selected randomly by stratified sampling from medical examination center of the hospital during synchronization as control group. We detected Ureaplasma urealyticum, DNA quantitative expression of cervical secretions from patients with CIN or cervical cancer by means of Fluorescent quantitative PCR(FQ-PCR) and also HPV DNA by both Hybrid capture Ⅱ and flow-through hybridization and gene chip. Results The positive rate of UU in case group was 68. 5% , in control group was 38.7%. Compared positive rate of UU between case group and control group, the difference had statistical significance. In 124 cases detected for both UU DNA and HPV DNA, 58.9% were positive for both UU DNA and HPV DNA. Conclusion There may be some relationship between UU positive of lower genital tract and cervical intra-epithelial neoplasia or cervical cancer, especially there may be some relationship between UU positive and high-grade lesion of cervix.There maybe interaction between UU and infection of HPV.