中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2011年
3期
269-272
,共4页
陈葆国%郑瑞%颜卫华%陈秋月%石卫武%李伯利
陳葆國%鄭瑞%顏衛華%陳鞦月%石衛武%李伯利
진보국%정서%안위화%진추월%석위무%리백리
脑梗%颈动脉粥样硬化%CD4+CD28null%T细胞%CD158j%磷酸化ERK
腦梗%頸動脈粥樣硬化%CD4+CD28null%T細胞%CD158j%燐痠化ERK
뇌경%경동맥죽양경화%CD4+CD28null%T세포%CD158j%린산화ERK
Cerebral infarction%Carotid atherosclerosis%CD4+CD28null T cells%CD158j%p-ERK
目的 探讨伴颈动脉粥样硬化(CA)脑梗患者CD4+CD28null T细胞上CD158j的表达百分率和ERK磷酸化(p-ERK)水平的关系及对颈动脉粥样斑块不稳定的影响.方法 采用流式细胞术检测106例伴CA脑梗、33例颈动脉正常脑梗患者和50例健康人外周血CD4+CD28null T细胞数量,以及CD4+CD28null T细胞CD158j和穿孔素(perforin)的表达,36例斑块不稳定患者CD4+T细胞p-ERK水平.ELISA法检测血清IFN-γ水平.B超检查以上人群颈动脉血管内壁粥样硬化情况.结果 脑梗患者CD4+CD28nullT细胞数量、CD4+CD28null T细胞CD158j和perforin的表达,以及血清IFN-γ水平,均明显高于健康对照组(P均<0.01);CD4+CD28null T细胞数量、CD4+CD28null T细胞CD158j和perforin的表达,以及血清IFN-γ水平,在脑梗患者中由高到低依次为颈动脉不稳定斑块组、颈动脉稳定斑块组、颈动脉内-中膜厚度(IMT)增厚组和颈动脉正常组.颈动脉不稳定斑块脑梗患者CD4+CD28null T细胞CD158j的表达与p-ERK水平呈显著性正相关(P<0.01).结论 伴CA脑梗患者CD4+CD28null T细胞数量明显增多.CD158j可能通过上调p-ERK水平,促进CD4+CD28null淋巴细胞增殖,产生细胞毒效应和分泌细胞因子,最终导致颈动脉粥样斑块不稳定.
目的 探討伴頸動脈粥樣硬化(CA)腦梗患者CD4+CD28null T細胞上CD158j的錶達百分率和ERK燐痠化(p-ERK)水平的關繫及對頸動脈粥樣斑塊不穩定的影響.方法 採用流式細胞術檢測106例伴CA腦梗、33例頸動脈正常腦梗患者和50例健康人外週血CD4+CD28null T細胞數量,以及CD4+CD28null T細胞CD158j和穿孔素(perforin)的錶達,36例斑塊不穩定患者CD4+T細胞p-ERK水平.ELISA法檢測血清IFN-γ水平.B超檢查以上人群頸動脈血管內壁粥樣硬化情況.結果 腦梗患者CD4+CD28nullT細胞數量、CD4+CD28null T細胞CD158j和perforin的錶達,以及血清IFN-γ水平,均明顯高于健康對照組(P均<0.01);CD4+CD28null T細胞數量、CD4+CD28null T細胞CD158j和perforin的錶達,以及血清IFN-γ水平,在腦梗患者中由高到低依次為頸動脈不穩定斑塊組、頸動脈穩定斑塊組、頸動脈內-中膜厚度(IMT)增厚組和頸動脈正常組.頸動脈不穩定斑塊腦梗患者CD4+CD28null T細胞CD158j的錶達與p-ERK水平呈顯著性正相關(P<0.01).結論 伴CA腦梗患者CD4+CD28null T細胞數量明顯增多.CD158j可能通過上調p-ERK水平,促進CD4+CD28null淋巴細胞增殖,產生細胞毒效應和分泌細胞因子,最終導緻頸動脈粥樣斑塊不穩定.
목적 탐토반경동맥죽양경화(CA)뇌경환자CD4+CD28null T세포상CD158j적표체백분솔화ERK린산화(p-ERK)수평적관계급대경동맥죽양반괴불은정적영향.방법 채용류식세포술검측106례반CA뇌경、33례경동맥정상뇌경환자화50례건강인외주혈CD4+CD28null T세포수량,이급CD4+CD28null T세포CD158j화천공소(perforin)적표체,36례반괴불은정환자CD4+T세포p-ERK수평.ELISA법검측혈청IFN-γ수평.B초검사이상인군경동맥혈관내벽죽양경화정황.결과 뇌경환자CD4+CD28nullT세포수량、CD4+CD28null T세포CD158j화perforin적표체,이급혈청IFN-γ수평,균명현고우건강대조조(P균<0.01);CD4+CD28null T세포수량、CD4+CD28null T세포CD158j화perforin적표체,이급혈청IFN-γ수평,재뇌경환자중유고도저의차위경동맥불은정반괴조、경동맥은정반괴조、경동맥내-중막후도(IMT)증후조화경동맥정상조.경동맥불은정반괴뇌경환자CD4+CD28null T세포CD158j적표체여p-ERK수평정현저성정상관(P<0.01).결론 반CA뇌경환자CD4+CD28null T세포수량명현증다.CD158j가능통과상조p-ERK수평,촉진CD4+CD28null림파세포증식,산생세포독효응화분비세포인자,최종도치경동맥죽양반괴불은정.
Objective To investigate the relationship between CD158j expression and phosphorylated ERK (p-ERK) in CD4+ CD28null T cells in cerebral infarction (CI) patients- with carotid atherosclerosis and its effects on carotid atherosclerotic plaque stability. Methods Percentage of peripheral CD4+ CD28null and the expression of CD158j and perform on CD4+ CD28null cells was analyzed with flow cytometry in 106 CI patients with carotid atherosclerosis, 33 CI patients with normal carotid arteries and in 50 normal controls, respectively; p-ERK expression was assayed with flow cytometry in 36 CI patients with unstable plaque, and serum IFN-γ was detected with ELISA. The intima-media thickness (IMT) of bilateral carotid arteries in all subjects was confirmed by the colour Doppler ultrasonograph imagingResults Percentage of the CD4+ CD28null T cells, expression of CD158j and perform on CD4+ CD28null T cells and the serum IFN-γ levels was dramatically higher in CI patients than that in normal controls, respectively (all P <0.01), which was decreased in an order of CI patients with patients with unstable plaque, stable plaque, carotid artery IMT and with normal carotid artery. A strong positive correlation was observed between the CD158j expression and degree of p-ERK in CI patients with unstable plaque (P < 0. 01). Conclusion CD4+ CD28null T cells were significantly increased in CI patients with carotid atherosclerosis. CD158j might up-regulate p-ERK expression and induce the proliferation of the CD4+ CD28nullT cells; consequently, higher cytokine production such as IFN-γ produced by CD4+ CD28null T cells may cause the formation of unstable plaque.