中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2011年
5期
448-451
,共4页
丙型肝炎%髓样树突状细胞%浆样树突状细胞%流式细胞术
丙型肝炎%髓樣樹突狀細胞%漿樣樹突狀細胞%流式細胞術
병형간염%수양수돌상세포%장양수돌상세포%류식세포술
Hepatitis C%Myeloid dendritic cells%Plasmacytoid dendritic cells%Flow cytometry
目的 探讨慢性HCV感染者外周血中髓样树突状细胞(mDC)和浆样树突状细胞(pDC)频数和表型的变化,并分析其与丙型肝炎临床指标间的相关性.方法 采用流式细胞术检测HCV感染者及健康对照外周血中mDC和pDC的频数及细胞表面共刺激分子HLA-DR、CD83、CD86、CD40和共抑制分子PD-L1的表达水平,并分析DC频数与HCV感染者血浆病毒载量、谷丙转氨酶(ALT)的相关性.结果 与健康对照组相比,HCV感染者外周血中mDC和pDC的频数明显降低(患者组分别为0.37±0.19和0.19±0.12,对照组为0.51±0.18和0.29±0.13,P＜0.05),且mDC频数与血浆HCV载量和血清ALT水平呈负相关(r=-0.5878,P＜0.0001;r=-0.4628,P=0.003).患者mDC和pDC表面共刺激分子HLA-DR、CD83、CD86、CD40以及共抑制分子PD-L1的表达均有不同程度升高,差别有统计学意义(共刺激分子P＜0.01,共抑制分子P＜0.05或0.01).结论 慢性HCV感染者外周血mDC和pDC频数下降,但DC表面共刺激分子和共抑制分子的表达均明显升高.该结果提示mDC数量的减少可能与HCV的慢性持续性感染有关.
目的 探討慢性HCV感染者外週血中髓樣樹突狀細胞(mDC)和漿樣樹突狀細胞(pDC)頻數和錶型的變化,併分析其與丙型肝炎臨床指標間的相關性.方法 採用流式細胞術檢測HCV感染者及健康對照外週血中mDC和pDC的頻數及細胞錶麵共刺激分子HLA-DR、CD83、CD86、CD40和共抑製分子PD-L1的錶達水平,併分析DC頻數與HCV感染者血漿病毒載量、穀丙轉氨酶(ALT)的相關性.結果 與健康對照組相比,HCV感染者外週血中mDC和pDC的頻數明顯降低(患者組分彆為0.37±0.19和0.19±0.12,對照組為0.51±0.18和0.29±0.13,P＜0.05),且mDC頻數與血漿HCV載量和血清ALT水平呈負相關(r=-0.5878,P＜0.0001;r=-0.4628,P=0.003).患者mDC和pDC錶麵共刺激分子HLA-DR、CD83、CD86、CD40以及共抑製分子PD-L1的錶達均有不同程度升高,差彆有統計學意義(共刺激分子P＜0.01,共抑製分子P＜0.05或0.01).結論 慢性HCV感染者外週血mDC和pDC頻數下降,但DC錶麵共刺激分子和共抑製分子的錶達均明顯升高.該結果提示mDC數量的減少可能與HCV的慢性持續性感染有關.
목적 탐토만성HCV감염자외주혈중수양수돌상세포(mDC)화장양수돌상세포(pDC)빈수화표형적변화,병분석기여병형간염림상지표간적상관성.방법 채용류식세포술검측HCV감염자급건강대조외주혈중mDC화pDC적빈수급세포표면공자격분자HLA-DR、CD83、CD86、CD40화공억제분자PD-L1적표체수평,병분석DC빈수여HCV감염자혈장병독재량、곡병전안매(ALT)적상관성.결과 여건강대조조상비,HCV감염자외주혈중mDC화pDC적빈수명현강저(환자조분별위0.37±0.19화0.19±0.12,대조조위0.51±0.18화0.29±0.13,P＜0.05),차mDC빈수여혈장HCV재량화혈청ALT수평정부상관(r=-0.5878,P＜0.0001;r=-0.4628,P=0.003).환자mDC화pDC표면공자격분자HLA-DR、CD83、CD86、CD40이급공억제분자PD-L1적표체균유불동정도승고,차별유통계학의의(공자격분자P＜0.01,공억제분자P＜0.05혹0.01).결론 만성HCV감염자외주혈mDC화pDC빈수하강,단DC표면공자격분자화공억제분자적표체균명현승고.해결과제시mDC수량적감소가능여HCV적만성지속성감염유관.
Objective To explore the frequencies and phenotype of myeloid and plasmacytoid dendritic cells (mDC and pDC) in chronic HCV infection and to investigate the relationships between DC frequencies and HCV viral load and serum ALT level. Methods PBMC were isolated from chronic HCV infected patients and healthy control. Multi-color flow cytometry was used to analyze the frequencies and surface marker expression on mDC and pDC. The relationship between DC frequencies and viral load and ALT level was also calculated. Results In comparison with healthy control, frequencies of mDC and pDC in chronic HCV infection were significantly decreased (0. 37 ± 0. 19 and 0. 19 ± 0. 12 vs 0. 51 ± 0. 18 and 0. 29 ± 0.13, P＜0.05). The frequency of mDC was negatively correlated with HCV viral load (r= -0.5878, P ＜ 0. 0001 ) and serum ALT level ( r = - 0. 4628 , P = 0. 003 ). Both costimulatory markers ( HLA-DR, CD83, CD86, and CD40) and coinhibitory marker (PD-L1) expression on mDC and pDC in HCV infection were increased (P＜0.01 for costimulatory marker, P＜0.05 or F＜0.01 for coinhibitory marker). Conclusion The frequencies of mDC and pDC in chronic HCV infection were decreased, while the expression of costimulatory markers and coinhibitory marker were increased or not decreased in HCV infection. The decreased frequency of mDC was probably related to persistance of HCV infection.
