CAJ | 학술논문

目的通过对体外培养前脂肪细胞并分别与聚乳酸-羟基乙酸共聚物(PLGA)支架、胶原支架以及透明质酸支架复合,研究3种不同支架材料的细胞相容性.方法切取成年女性腹部皮下脂肪组织,采用胶原酶消化的方法分离培养人前脂肪细胞,采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑臭盐(MTT)法观察3种不同支架材料的细胞相容性,并以透明质酸作为支架,考察静态和水平摇床两种接种培养方式对前脂肪细胞在支架上接种率的影响.结果前脂肪细胞可以在体外成功地分离、培养,能定向分化为脂肪细胞,并可以进行传代扩增.前脂肪细胞在PLGA、胶原以及透明质酸3种材料上生长良好,其中透明质酸支架以及水平摇床接种培养效果更佳.结论透明质酸更加适宜作为组织工程化脂肪的支架材料,水平摇床的接种培养可提高细胞与支架材料之间的接种率,优于静态培养.
목적 통과대체외배양전지방세포병분별여취유산-간기을산공취물(PLGA)지가、효원지가이급투명질산지가복합,연구3충불동지가재료적세포상용성.방법 절취성년녀성복부피하지방조직,채용효원매소화적방법분리배양인전지방세포,채용3-(4,5-이갑기새서-2)-2,5-이분기사담서취염(MTT)법관찰3충불동지가재료적세포상용성,병이투명질산작위지가,고찰정태화수평요상량충접충배양방식대전지방세포재지가상접충솔적영향.결과 전지방세포가이재체외성공지분리、배양,능정향분화위지방세포,병가이진행전대확증.전지방세포재PLGA、효원이급투명질산3충재료상생장량호,기중투명질산지가이급수평요상접충배양효과경가.결론 투명질산경가괄의작위조직공정화지방적지가재료,수평요상적접충배양가제고세포여지가재료지간적접충솔,우우정태배양.
Objective To culture preadipocytes in vitro and to study the cell compatibility of PLGA scaffolds,collagen scarfolds and hyaluronic acid-based scaffolds and tO choose the optimal seeding method.Methods The preadipocytes from human abdominal adipose tissue were isolated and cultured in enzyme-digesting method.The generation of human preadipocytes was planted on PLGA scaffolds,collagen scaffolds and hyaluronic acid-based scaffolds.and the cell compatibility was observed by MTT method.The seeding efficiency of human preadipocytes on scaffolds.human preadipocytes were seeded to hyaluronic acid-based scaffolds by static culture and stirred culture.Results Compared compatibility of preadipocyte with three different scaffolds,there was great difference between hyaluronic acid-based scaffolds and PLGA scaffolds.Difference also existed between hyaluronic acid-based scaffolds and collagen scaffolds that were different from PLGA scaffolds.Among them,hyaluronic acid-based scaffolds was the best.Conclusion Hyaluronic acid iS a better scaffolds material for adipose tissue engineering compared with PLGA and collagen.The seeding efficiency of stirred culture is higher than static culture,which is an optimal method for cell seeding tO 3-D scaffolds.