CAJ | 학술논문

目的探讨同种异体羊膜材料复合应用神经生长因子替代神经材料桥接周围神经缺损的可行性.方法48只健康SD大鼠制备坐骨神经缺损模型,随机分为4组,即自体神经原位移植组(A组),异体神经移植应用环孢素A(Cyclosporine A,CSA)5 mg/(kg.d)5周组(B组),异体羊膜材料桥接缺损复合应用神经生长因子组(C组),单纯异体神经移植组(D组).6只Wistar大鼠取双侧坐骨神经作为供体.于术后12周取移植段神经行光镜、电镜形态学观察,测定神经运动诱发电位潜伏期、神经运动诱发电位峰值、神经传导速度和振幅积分、腓肠肌最大收缩力,再生轴突计数及髓鞘厚度测定.结果12周时A、B、C组的各项检测指标明显优于D组(潜伏期、诱发电位峰值、传导速度、腓肠肌最大收缩力、髓鞘厚度及再生轴突计数ANOVA结果分别为F=12.87,P＜0.05;F=19.54,P＜0.05;F=35.21,P＜0.01;F=56.33,P＜0.01.F=75.26,P＜0.05;F=14.83,P＜0 05;F=96.11,P＜0.01),3组间差异无显著性(P＞0.05),再生神经形态与功能恢复良好.结论对于长段周围神经缺损,应用异体羊膜复合神经生长因子进行桥接可以有效促进神经再生及功能恢复.
목적탐토동충이체양막재료복합응용신경생장인자체대신경재료교접주위신경결손적가행성.방법48지건강SD대서제비좌골신경결손모형,수궤분위4조,즉자체신경원위이식조(A조),이체신경이식응용배포소A(Cyclosporine A,CSA)5 mg/(kg.d)5주조(B조),이체양막재료교접결손복합응용신경생장인자조(C조),단순이체신경이식조(D조).6지Wistar대서취쌍측좌골신경작위공체.우술후12주취이식단신경행광경、전경형태학관찰,측정신경운동유발전위잠복기、신경운동유발전위봉치、신경전도속도화진폭적분、비장기최대수축력,재생축돌계수급수초후도측정.결과12주시A、B、C조적각항검측지표명현우우D조(잠복기、유발전위봉치、전도속도、비장기최대수축력、수초후도급재생축돌계수ANOVA결과분별위F=12.87,P＜0.05;F=19.54,P＜0.05;F=35.21,P＜0.01;F=56.33,P＜0.01.F=75.26,P＜0.05;F=14.83,P＜0 05;F=96.11,P＜0.01),3조간차이무현저성(P＞0.05),재생신경형태여공능회복량호.결론대우장단주위신경결손,응용이체양막복합신경생장인자진행교접가이유효촉진신경재생급공능회복.
Aim To investigate the feasibility of using arnnion allograftcombined with nerve growth factor to bridge peripheral nerve deficit.Methods 48 SD rats as models of sciatic nerve deficit were randomlyassigned into one of the following groups: autograft (Group A), allograftwith Cyclosporine A (CSA) 5 mg/(kg @ d) for 5 weeks (Group B), deficitbridged by amnion allograft combined with nerve growth factor (Group C) ,and allograft with no immune treatment (Group D) . 6 Wistar rats weresacrificed as the donors of the sciatic nerve allograft. Observation wascarried under light microscope and electron microscope examination at 12weeks post-operatively for morphological studying; The latency, ampli-tude, conduct velocity and negative area under the curve were recordedas electrophysiologic index; Maximum contractility of gastrocnemiusmuscle and thickness of myelin of the regenenative nerve were measured;Axons counting were also performed to evaluate the quality of regenera-tion. Results At 12 weeks, group A, B and C recovered better (Indexessuch as latency, amplitude, conduct velocity , negative area under thecurve, maximum contractility of gastrocnemius muscle, thickness of myelinand axons counting were compared by ANOVA test, F= 12.87, P ＜0.05; F=19.54, P ＜0.05; F=35.21, P ＜0.01; F=56.33, P ＜0.01. F=75.26, P ＜0.05; F=14.83, P ＜0.05; F=96.11, P ＜0.01), and no significant difference was found among these groups ( P ＞0.05). Conclusion For long distance peripheral nerve deficit, usingamnion allograft combined with nerve growth factor to bridge the deficit canfacilitate the regeneration of nerve and the recovery of function.