CAJ | 학술논문

近年来,危害小黑杨Populus simonii×P.nigra的害虫发生严重,给林业生产造成很大损失.为了提高小黑杨的抗虫能力,避免使用杀虫剂带来的污染,用农杆菌介导法将澳大利亚漏斗蛛Atrax robustus的毒蛋白基因和苏云金芽孢杆菌Cry Ⅰ A(b)基因C末端的融合基因BGT转化入小黑杨.PCR和Southern印记分析转基因植株,结果表明,BGT杀虫基因已经整合在小黑杨基因组上.活性实验表明,取食转基因杨树6天和9天后,舞毒蛾Lymantria dispar 2龄幼虫的校正死亡率分别是37.0%和92.6%.方差分析表明取食转基因和对照杨树的舞毒蛾幼虫体重差异显著.这些结果显示转基因杨树上的舞毒蛾的发育速率受到影响.
근년래,위해소흑양Populus simonii×P.nigra적해충발생엄중,급임업생산조성흔대손실.위료제고소흑양적항충능력,피면사용살충제대래적오염,용농간균개도법장오대리아루두주Atrax robustus적독단백기인화소운금아포간균Cry Ⅰ A(b)기인C말단적융합기인BGT전화입소흑양.PCR화Southern인기분석전기인식주,결과표명,BGT살충기인이경정합재소흑양기인조상.활성실험표명,취식전기인양수6천화9천후,무독아Lymantria dispar 2령유충적교정사망솔분별시37.0%화92.6%.방차분석표명취식전기인화대조양수적무독아유충체중차이현저.저사결과현시전기인양수상적무독아적발육속솔수도영향.
In recent years, the pest insects on hybrid poplar Populus simonii × P. nigra broke out heavily, which caused great losses in forestry. In order to improve insect resistance of P. simonii × P. nigra and avoid pollution due to insecticides, the fused BGT gene consisting of the insecticidal toxin gene from the spider, Atrax robustus, and the C terminal of Cry Ⅰ A (b) gene from Bacillus thuringiensis was transferred into P. simonii × P. nigra by Agrobacteriumm-mediated transformation system. The results of PCR and Southern blotting analyses showed that the insecticidal gene of BGT was integrated into the genome of P. simonii × P. nigra. The corrected mortality of the second instar of Lymantria dispar in 6 days and 9 days after they were fed with the transgenic poplars was 37.0% and92.6% , respectively. Analysis of variance showed that there was significant difference in body weight between L. dispar larvae fed with the transformed poplars and those fed with untransformed poplars. The results indicated that the growth rate of L. dispar fed with the transgenic poplars was affected negatively.