背景:电化学治疗是治疗肿瘤的一种新的而有效的工具.这种技术非常容易控制和操作,而且对正常组织副作用小.特别对治疗浅表肿瘤非常有效.在电化学治疗治疗过程中,电磁脉冲参数的选取与构成是非常关键的.目的:利用电化学治疗植入S-180肉瘤的昆明鼠,获得电磁脉冲治疗的最佳参数.单位:四川大学电子信息学院无线电物理教研室.设计:随机分组设计、对照实验.材料:实验于2003-01/2004-05在四川大学生命科学学院细胞实验室完成.选择昆明鼠106只随机分为12组,电压500V、脉冲数为5、电容分别为6,10,14μF组各9只,电压700V、脉冲数为5、电容分别为6,10,14μF组各9只,电压900V、脉冲数为5、电容分别为6,10,14μF组各9只,电压700 V、电容10 μF、脉冲数分别为7,9组各8只,对照组9只.方法:博来霉素多点腹腔注射入肿瘤块(0.04 mg/只),用针状辐射器加上电磁脉冲,每隔2 d治疗1次,共3次,18 d以后处死小鼠.照射前和照射后第10,18天,测量肿瘤体积,公式为肿瘤体积(mm3)=3.141 59×长×宽×高/6.抑制率(%)=(对照组平均肿瘤体积-照射后平均肿瘤体积)/对照组平均肿瘤体积×100%.相对生长速率=照射后肿瘤体积/照射前肿瘤体积.主要观察指标:肿瘤体积;抑制率;相对生长速率.结果:纳入动物106只,均进入结果分析.①当电容是6 μF,脉冲数是5而电压变化时[以第10天为例,电压为500,700,900V时肿瘤体积分别为(66.99±91.17),(62.58±71.83),(78.43±73.91)mm3],电压700 V时总体效果最好,而电压900 V时总体效果较差.②当电压和脉冲数固定而电容变化时(以电压700 V,脉冲数为5为例,电容为6,10,14 μF时肿瘤抑制率分别为92.14%,90.90%,84.97%),电容6 μF时效果最好,电容14 μF时效果最差.③当电压和电容固定,而脉冲数在变化时[以电压700 V,电容10μF,第10天为例,脉冲数为5,7,9时肿瘤体积分别为(80.66±38.17),(41.33±36.40),(39.86±23.03)mm3],随着脉冲数的增加抑制效果变好,而脉冲数为9时最好.结论:通过实验获得电磁脉冲的最佳参数:当脉冲数是5,电压700 V,电容6μF时,抑制肿瘤的效果是最好的;当电压固定在700 V,电容固定在10μF时,抑制肿瘤的效果随着脉冲数的增长而增长,并且最好的脉冲数是9.
揹景:電化學治療是治療腫瘤的一種新的而有效的工具.這種技術非常容易控製和操作,而且對正常組織副作用小.特彆對治療淺錶腫瘤非常有效.在電化學治療治療過程中,電磁脈遲參數的選取與構成是非常關鍵的.目的:利用電化學治療植入S-180肉瘤的昆明鼠,穫得電磁脈遲治療的最佳參數.單位:四川大學電子信息學院無線電物理教研室.設計:隨機分組設計、對照實驗.材料:實驗于2003-01/2004-05在四川大學生命科學學院細胞實驗室完成.選擇昆明鼠106隻隨機分為12組,電壓500V、脈遲數為5、電容分彆為6,10,14μF組各9隻,電壓700V、脈遲數為5、電容分彆為6,10,14μF組各9隻,電壓900V、脈遲數為5、電容分彆為6,10,14μF組各9隻,電壓700 V、電容10 μF、脈遲數分彆為7,9組各8隻,對照組9隻.方法:博來黴素多點腹腔註射入腫瘤塊(0.04 mg/隻),用針狀輻射器加上電磁脈遲,每隔2 d治療1次,共3次,18 d以後處死小鼠.照射前和照射後第10,18天,測量腫瘤體積,公式為腫瘤體積(mm3)=3.141 59×長×寬×高/6.抑製率(%)=(對照組平均腫瘤體積-照射後平均腫瘤體積)/對照組平均腫瘤體積×100%.相對生長速率=照射後腫瘤體積/照射前腫瘤體積.主要觀察指標:腫瘤體積;抑製率;相對生長速率.結果:納入動物106隻,均進入結果分析.①噹電容是6 μF,脈遲數是5而電壓變化時[以第10天為例,電壓為500,700,900V時腫瘤體積分彆為(66.99±91.17),(62.58±71.83),(78.43±73.91)mm3],電壓700 V時總體效果最好,而電壓900 V時總體效果較差.②噹電壓和脈遲數固定而電容變化時(以電壓700 V,脈遲數為5為例,電容為6,10,14 μF時腫瘤抑製率分彆為92.14%,90.90%,84.97%),電容6 μF時效果最好,電容14 μF時效果最差.③噹電壓和電容固定,而脈遲數在變化時[以電壓700 V,電容10μF,第10天為例,脈遲數為5,7,9時腫瘤體積分彆為(80.66±38.17),(41.33±36.40),(39.86±23.03)mm3],隨著脈遲數的增加抑製效果變好,而脈遲數為9時最好.結論:通過實驗穫得電磁脈遲的最佳參數:噹脈遲數是5,電壓700 V,電容6μF時,抑製腫瘤的效果是最好的;噹電壓固定在700 V,電容固定在10μF時,抑製腫瘤的效果隨著脈遲數的增長而增長,併且最好的脈遲數是9.
배경:전화학치료시치료종류적일충신적이유효적공구.저충기술비상용역공제화조작,이차대정상조직부작용소.특별대치료천표종류비상유효.재전화학치료치료과정중,전자맥충삼수적선취여구성시비상관건적.목적:이용전화학치료식입S-180육류적곤명서,획득전자맥충치료적최가삼수.단위:사천대학전자신식학원무선전물리교연실.설계:수궤분조설계、대조실험.재료:실험우2003-01/2004-05재사천대학생명과학학원세포실험실완성.선택곤명서106지수궤분위12조,전압500V、맥충수위5、전용분별위6,10,14μF조각9지,전압700V、맥충수위5、전용분별위6,10,14μF조각9지,전압900V、맥충수위5、전용분별위6,10,14μF조각9지,전압700 V、전용10 μF、맥충수분별위7,9조각8지,대조조9지.방법:박래매소다점복강주사입종류괴(0.04 mg/지),용침상복사기가상전자맥충,매격2 d치료1차,공3차,18 d이후처사소서.조사전화조사후제10,18천,측량종류체적,공식위종류체적(mm3)=3.141 59×장×관×고/6.억제솔(%)=(대조조평균종류체적-조사후평균종류체적)/대조조평균종류체적×100%.상대생장속솔=조사후종류체적/조사전종류체적.주요관찰지표:종류체적;억제솔;상대생장속솔.결과:납입동물106지,균진입결과분석.①당전용시6 μF,맥충수시5이전압변화시[이제10천위례,전압위500,700,900V시종류체적분별위(66.99±91.17),(62.58±71.83),(78.43±73.91)mm3],전압700 V시총체효과최호,이전압900 V시총체효과교차.②당전압화맥충수고정이전용변화시(이전압700 V,맥충수위5위례,전용위6,10,14 μF시종류억제솔분별위92.14%,90.90%,84.97%),전용6 μF시효과최호,전용14 μF시효과최차.③당전압화전용고정,이맥충수재변화시[이전압700 V,전용10μF,제10천위례,맥충수위5,7,9시종류체적분별위(80.66±38.17),(41.33±36.40),(39.86±23.03)mm3],수착맥충수적증가억제효과변호,이맥충수위9시최호.결론:통과실험획득전자맥충적최가삼수:당맥충수시5,전압700 V,전용6μF시,억제종류적효과시최호적;당전압고정재700 V,전용고정재10μF시,억제종류적효과수착맥충수적증장이증장,병차최호적맥충수시9.
BACKGROUND: Electrochemotherapy(ECT) is a new and an efficient approach for the treatment of tumor. The technique is very easy to control and operate and is of little harm to normal tissue. Especially, it is very efficient in curing the superficial tumors. In the treatment process of ECT, the selection and constitute of electromagnetic impulse are the key factors.OBJECTIVE: To use the ECT as a therapeutic method for S-180 sarcomas in Kunming mice and obtain the optimal parameters of electromagnetic impulse in curing.SETTING: Department of Wireless Physics, College of Electronics-Information, Sichuan University DESIGN: Random grouping design and controlled experiment study MATERIALS: This experiment was conducted at the Laboratory of Cells, College of Life Sciences, Sichuan University from January 2003 to May 2004. Totally 106 Kunming mice were randomly divided into 12 groups: voltage 500 V, pulse number 5 and capacitance 6,10 and 14 μF, respectively in 3 groups, with 9 mice in each group;voltage 700 V, pulse number 5 and capacitance 6,10 and 14 μF, respectively in 3 groups, with 9 mice in each group; voltage 900 V, pulse number 5 and capacitance 6,10 and 14 μF, respectively in 3 groups,with 9 mice in each group; voltage 700 V, capacitance 10 μF, pulse number 7 and and 9 respectively in 2 groups , with 8 mice in each group , and control group with 9 mice in it.METHODS: The treatment includes injection of Bleomycin 0.04 mg per one (intraperitoneal injecting into tumors through multiple points), followed by application of electromagnetic impulse using the needles. The treatment was taken every three days andthree times in total. Mice were euthanized 18 days later. Tumor volume was measured before irradiation and 10 and 18 days after irradiation. Tumor volume (mm3)=3.141 59×length×width×height/6. Inhibitory rate(%)=(average tumor volume of the control group-average tumor volume after irradiation)/average tumor volume of control group×100%. The relative growth velocity=tumor volume after irradiation/Tumor volume before irradiation.MAIN OUTCOME MEASURES: Tumor volume; inhibitory rate; relative growth velocity RESULTS: Totally 106 animals were enrolled in the experiment and all of them entered the stage of result analysis. ①When the capacitance was 6 μF, the impulse numbers are 5, the voltage altered [for example the 10th day, when voltage was 500, 700, 900 V, the tumor volume was (66.99±91.17),(62.58±71.83),(78.43±73.91) mm3 , respectively]. The effect was the best when the voltage was 700 V, and was the worst when 900 V).②When the voltage and capacitance were fixed and the pulse numbers altered, for example voltage 700 V and capacitance 10 μF, taking the 10th day as the example , the pulse number was 5, 7 and 9, the tumor volume was (80.66±38.17),(41.33±36.40),(39.86±23.03) mm3, respectively. The inhibitory effect was better with the increase of pulse number, and the best when the pulse number was 9.CONCLUSION: According to experimental results, following parameters of electromagnetic impulse, can be concluded: ①The inhibitory effect is the best when the number of impulse is 5, voltage 700 V, capacitance 6 μF. ②The inhibitory effect increases with the increase of pulse number when the voltage is 700 V and capacitance is 10 μF, and is the best when the pulse number is 9.
