检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2009年
15期
1224-1225,1227
,共3页
血小板%温度%血小板聚集%低温保存
血小闆%溫度%血小闆聚集%低溫保存
혈소판%온도%혈소판취집%저온보존
platelets%temperature%platelet aggregation%cryopreservation
目的 探讨冰冻血小板保存温度波动与冰冻血小板不可逆聚集情况发生的相关性.方法 对2006～2008年共1 842袋冰冻血小板在保存期间不同温度波动范围与融化后发生血小板不可逆聚集的情况进行统计分析.结果 冰冻血小板保存温度分别在-80～-60 ℃和-80～-50 ℃范围波动,冰冻血小板融化复苏后血小板不可逆聚集发生率分别为6.39%和31.21%,保存温度在-80～-70 ℃范围波动的对照组血小板不可逆聚集发生率为2.25%,前两种保存温度与对照组比较,组间差异均有统计学意义(P＜0.01);保存温度在-80～-50 ℃范围波动,手采和单采两种冰冻血小板融化复苏后,单采冰冻血小板不可逆聚集的发生率为72.97%,手采冰冻血小板不可逆聚集为18.33%,二者的发生率差异有统计学意义 (χ2=39.33,P＜0.01);保存温度在-80～-60 ℃范围波动,单采与手采两种冰冻血小板比较,差异无统计学意义(χ2=0.89,P＞0.05).结论 当冰冻血小板保存温度分别在-80～-60 ℃和-80～-50 ℃波动时,融化复苏后血小板不可逆聚集有较高的发生率,在-80～-70 ℃范围波动时,血小板不可逆聚集发生率较低.-80～-70 ℃的温度范围可以作为目前采供血机构保存冰冻血小板选择温度条件的参考.
目的 探討冰凍血小闆保存溫度波動與冰凍血小闆不可逆聚集情況髮生的相關性.方法 對2006～2008年共1 842袋冰凍血小闆在保存期間不同溫度波動範圍與融化後髮生血小闆不可逆聚集的情況進行統計分析.結果 冰凍血小闆保存溫度分彆在-80～-60 ℃和-80～-50 ℃範圍波動,冰凍血小闆融化複囌後血小闆不可逆聚集髮生率分彆為6.39%和31.21%,保存溫度在-80～-70 ℃範圍波動的對照組血小闆不可逆聚集髮生率為2.25%,前兩種保存溫度與對照組比較,組間差異均有統計學意義(P＜0.01);保存溫度在-80～-50 ℃範圍波動,手採和單採兩種冰凍血小闆融化複囌後,單採冰凍血小闆不可逆聚集的髮生率為72.97%,手採冰凍血小闆不可逆聚集為18.33%,二者的髮生率差異有統計學意義 (χ2=39.33,P＜0.01);保存溫度在-80～-60 ℃範圍波動,單採與手採兩種冰凍血小闆比較,差異無統計學意義(χ2=0.89,P＞0.05).結論 噹冰凍血小闆保存溫度分彆在-80～-60 ℃和-80～-50 ℃波動時,融化複囌後血小闆不可逆聚集有較高的髮生率,在-80～-70 ℃範圍波動時,血小闆不可逆聚集髮生率較低.-80～-70 ℃的溫度範圍可以作為目前採供血機構保存冰凍血小闆選擇溫度條件的參攷.
목적 탐토빙동혈소판보존온도파동여빙동혈소판불가역취집정황발생적상관성.방법 대2006～2008년공1 842대빙동혈소판재보존기간불동온도파동범위여융화후발생혈소판불가역취집적정황진행통계분석.결과 빙동혈소판보존온도분별재-80～-60 ℃화-80～-50 ℃범위파동,빙동혈소판융화복소후혈소판불가역취집발생솔분별위6.39%화31.21%,보존온도재-80～-70 ℃범위파동적대조조혈소판불가역취집발생솔위2.25%,전량충보존온도여대조조비교,조간차이균유통계학의의(P＜0.01);보존온도재-80～-50 ℃범위파동,수채화단채량충빙동혈소판융화복소후,단채빙동혈소판불가역취집적발생솔위72.97%,수채빙동혈소판불가역취집위18.33%,이자적발생솔차이유통계학의의 (χ2=39.33,P＜0.01);보존온도재-80～-60 ℃범위파동,단채여수채량충빙동혈소판비교,차이무통계학의의(χ2=0.89,P＞0.05).결론 당빙동혈소판보존온도분별재-80～-60 ℃화-80～-50 ℃파동시,융화복소후혈소판불가역취집유교고적발생솔,재-80～-70 ℃범위파동시,혈소판불가역취집발생솔교저.-80～-70 ℃적온도범위가이작위목전채공혈궤구보존빙동혈소판선택온도조건적삼고.
Objective Discussion on the relevance of the frozen platelet preservation during temperature fluctuations with the frozen platelet occurrence irreversible aggregation.Methods During 2006-2008,a total of 1 842 bags of frozen platelets preservation and melting data were statistically analysed,and the relevance of the frozen platelets preservation during temperature fluctuations with the occurrence of platelet irreversible aggregation after resuscitative melting were investigated.Results The frozen platelets storage temperature between -80 ℃ and -60 ℃ and -80 ℃ and -50 ℃ range of fluctuation,the platelets after melt resuscitative occurrence irreversible aggregation were 6.39% and 31.21%.Compared the two observation groups with the control group(preservation temperature between -80 ℃ and -70 ℃ range of fluctuation),there were significant differences(P＜0.01).Under the preservation temperature between -80 ℃ and -50 ℃ range of fluctuation,apheresis platelets and concentrate platelets after melt resuscitative,apheresis platelet occurrence irreversible aggregation was 72.97%,the concentrate platelet occurrence irreversible aggregation was 18.33%. Compared the two groups,there were significant difference (χ2=39.33,P＜0.01);under preservation temperature between -80 ℃ and -60 ℃ range of fluctuation,apheresis platelet and concentrate platelet after melt resuscitative,there were no significant difference(χ2=0.89,P＞0.05).Conclusion Under preservation temperature between -80 ℃ and -60 ℃ and between -80 ℃ and -50 ℃ range of fluctuation,the platelet after melt resuscitative occurrence irreversible aggregation,incidence could occur often.Under preservation temperature between -80 ℃ and -70 ℃ range of fluctuation,the platelet after melt resuscitative occurrence irreversible aggregation,there is few incidence.Temperature range between -80 ℃ and -70 ℃ can be used as the current Blood Bank of frozen platelet reference temperature choice.
