CAJ | 학술논문

以杂交一代品种雪山椰菜花和自交系SZ-70为供试材料,研究了不同平阳霉素处理浓度对种子萌发的影响、不同激素种类和浓度对不定芽诱导和增殖培养的效果以及不同平阳霉素处理浓度对离体培养茎尖存活的影响.结果表明:以6-BA 2 mg/L和NAA 0.25 mg/L诱导茎尖产生不定芽的效果优于子叶和下胚轴;6-BA0.2 mg/L+NAA 0.02 mg/L+MET 0.2 mg/L对不定芽的增殖效果最佳;平阳霉素处理对种子的萌发率影响不明显,处理浓度越高,种子萌发后真叶抽出率越低;随着平阳霉素处理浓度的提高,茎尖存活率下降,且异常苗和玻璃苗增加.平阳霉素80 mg/L、30 min的处理对茎尖的诱变效果较为适合.
이잡교일대품충설산야채화화자교계SZ-70위공시재료,연구료불동평양매소처리농도대충자맹발적영향、불동격소충류화농도대불정아유도화증식배양적효과이급불동평양매소처리농도대리체배양경첨존활적영향.결과표명:이6-BA 2 mg/L화NAA 0.25 mg/L유도경첨산생불정아적효과우우자협화하배축;6-BA0.2 mg/L+NAA 0.02 mg/L+MET 0.2 mg/L대불정아적증식효과최가;평양매소처리대충자적맹발솔영향불명현,처리농도월고,충자맹발후진협추출솔월저;수착평양매소처리농도적제고,경첨존활솔하강,차이상묘화파리묘증가.평양매소80 mg/L、30 min적처리대경첨적유변효과교위괄합.
The F_1 hybrid Xueshan Yecaihua(SN)and the lnbred line SZ-70 bred by the Horticultural Research Institute were used as test materials,and the effects of Pingyangmicin and hormone treatments were studied on the seed germination,adventitious bud induction and propagation,and in vitro culture of stem apexes of B.oleracea vat.botrytis.The results showed that the stem apexes of sterilized seedlings were better than the cotyledons and hypocotyls of them in inducing adventitious buds with 2 mg/L 6-BA and 0.25 mg/L NAA.The medium added with 0.2 mg/L 6-BA,0.02 mg/L NAA and 0.2 mg/L MET had the best effect on the adventitious bud propagation.Pingyangmicin treatments had not an obvious effect on the seed germination,but increasing Pingyangmicin concentration could decrease the true leaf germination rate and the survival rate of the stem apexes and increase the abnormal and vitreous shoots.The treatment with 80 mg/L Pingyangmicin for 30 min was the median lethal dose of the stem apexes.