CAJ | 학술논문

背景:内源性6-羟基多巴胺能参与氧化应激,N-乙酰半胱氨酸能有效抗氧化和清除自由基.目的:探讨6-羟基多巴胺对骨髓基质细胞的毒性作用及N-乙酰半胱氨酸对其的拮抗作用.方法:在体外培养SD大鼠骨髓基质细胞,取第3代骨髓基质细胞分别加入终浓度为0,0.05,0.1 g/L的6-羟基多巴胺和终浓度为0,0.075,0.3,1.2,4.8 g/L的N-乙酰半胱氨酸.结果与结论:MTT检测发现0.05和0.1 g/L 6-羟基多巴胺可以明显降低骨髓基质细胞的细胞活性,而加入6-羟基多巴胺的同时加入0.3 g/L N-乙酰半胱氨酸可以显著抑制6-羟基多巴胺的毒性作用.提示抗氧化剂N-乙酰半胱氨酸可以影响6-羟基多巴胺的作用.
배경:내원성6-간기다파알능삼여양화응격,N-을선반광안산능유효항양화화청제자유기.목적:탐토6-간기다파알대골수기질세포적독성작용급N-을선반광안산대기적길항작용.방법:재체외배양SD대서골수기질세포,취제3대골수기질세포분별가입종농도위0,0.05,0.1 g/L적6-간기다파알화종농도위0,0.075,0.3,1.2,4.8 g/L적N-을선반광안산.결과여결론:MTT검측발현0.05화0.1 g/L 6-간기다파알가이명현강저골수기질세포적세포활성,이가입6-간기다파알적동시가입0.3 g/L N-을선반광안산가이현저억제6-간기다파알적독성작용.제시항양화제N-을선반광안산가이영향6-간기다파알적작용.
BACKGROUND: 6-hydroxydopamine, as an endogenous toxic factor in the pathogenesis of Parkinson's disease, participates in oxidative stress. N-acetylcysteine resists oxidation and removes free radicals effectively.OBJECTIVE: To investigate the toxicity of 6-hydroxydopamine in bone marrow stromal cells and the antagonistic effect of N-acetylcysteine on it. METHODS: Bone marrow stromal cells of Sprague-Dawley rats were cultured in vitro. Bone marrow stromal cells of passage 3 were treated with 6-hydroxydopamine with the final concentrations of 0,0.05,0.1g/L and N-acetylcysteine with the final concentrations of 0, 0.075,0.3,1.2,4.8g/L, respectively.RESULTS AND CONCLUSION: MTT assay showed that 6-hydroxydopamine (0.05 and 0.1 g/L) significantly decreased the viability of bone marrow stromal cells. This toxic effect of 6-hydroxydopamine was significantly inhibited by 0.3 g/L N-acetylcysteine. It suggests that antioxidant N-acetylcysteine may affect the toxic action of 6-hydroxydopamine.