CAJ | 학술논문

目的探讨色氨酸羟化酶2(TPH2)基因rs1386494和G1463A单核苷酸多态性与云南地区汉族抑郁症患者的关联关系.方法采用病例对照的方法,选取云南地区102例汉族抑郁症患者为研究对象,102例汉族健康者为正常对照,运用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术,检测TPH2基因该两位点单核苷酸多态性,并结合汉密尔顿抑郁量表(HAMD)症状评分分析两位点多态性与云南汉族抑郁症症状特点的关系.结果 1.rsl386494位点的基因型频率和等位基因频率在患者组和对照组中差异有统计学意义(分别为X~2=4.300,P=0.038;X~2=4.067,P=0.044);A等位基因在对照组中的出现频率高于患者组(P=0.044).2.在所有样本中均未观察到G1463A多态性现象.3.rs1386494位点基因型与HAMD总分比较,差异无统计学意义(F=2.461,P=0.120).结论 TPH2基因rs1386494多态性可能与云南地区汉族抑郁症的发病有关联,A等位基因可能是抑郁症的保护基因.
목적 탐토색안산간화매2(TPH2)기인rs1386494화G1463A단핵감산다태성여운남지구한족억욱증환자적관련관계.방법 채용병례대조적방법,선취운남지구102례한족억욱증환자위연구대상,102례한족건강자위정상대조,운용취합매련식반응-한제성편단장도다태성(PCR-RFLP)기술,검측TPH2기인해량위점단핵감산다태성,병결합한밀이돈억욱량표(HAMD)증상평분분석량위점다태성여운남한족억욱증증상특점적관계.결과 1.rsl386494위점적기인형빈솔화등위기인빈솔재환자조화대조조중차이유통계학의의(분별위X~2=4.300,P=0.038;X~2=4.067,P=0.044);A등위기인재대조조중적출현빈솔고우환자조(P=0.044).2.재소유양본중균미관찰도G1463A다태성현상.3.rs1386494위점기인형여HAMD총분비교,차이무통계학의의(F=2.461,P=0.120).결론 TPH2기인rs1386494다태성가능여운남지구한족억욱증적발병유관련,A등위기인가능시억욱증적보호기인.
Objective To investigate the association of the two single nucleotide polymorphisms(SNPs) rs1386494 and G1463A of tryptophan hydroxylase 2 gene with depression in Yunnan Han population. Methods A case-control study Was designed by collecting 102 patients and 102 controls.Polymerase chain reaction-restriction fragment length polymorphisms(PCR-RFLP)technique Was used to detect the rs1386494 and G1463A polymorphisms.All patients were evaluated with Hamilton Rating Scale for Depression(HAMD),and scores were compared according to different genotype.Results 1.There were differences in genotype and allele frequency of rsl386494 in Yunnan Han population(X~2=4.300,P=0.038;X~2=4.067,P=0.044).The A allele frequency of rsl386494 in controls Was higher than in patients(P=0.044).2.No genotype of G1463A polymorphism was observed in all samples.3.No significant association genotype of rsl386494 with scores of HAMD Was observed(F=2.461,P=0.120).Conclusion The polymorphism of rsl386494 may be associated with the vulnerability of Yunnan Han population,and the A allele maybe the protective gene for depression.