CAJ | 학술논문

目的探讨ghrelin受体变化对大鼠术后小肠动力不足的影响.方法采用体外肌条实验观察在卡巴胆碱(10 nmol/L)存在和缺乏时,不同浓度的ghrelin(0、0.01、0.1、0.5、1.0 μmol/L)对肌条收缩力的影响.采用免疫组织化学方法和Western blot技术检测行端侧肠吻合的实验组大鼠和行假手术的对照组大鼠小肠肌层ghrelin受体(GHS-R1a)的表达情况.结果在体外,卡巴胆碱存在时,ghrelin能够增强小肠平滑肌肌条的收缩,不同浓度的ghrelin(0.1、0.5、1.0 μmoL/L)引起的收缩力差异具有统计学意义[(223±18)%、(245±22)%、(264±25)%,P<0.01].免疫组化染色显示,GHS-R1a主要分布于小肠肌层,实验组大鼠小肠环形肌和纵行肌中GHS-R1a表达均弱于对照组.Western blot结果显示,实验组小肠ghrelin受体表达量(0.51±0.02)明显低于对照组(0.71±0.01,P<0.01).结论术后小肠肌层ghrelin受体表达下调所导致的ghrelin效应减弱可能参与术后小肠动力不足的发生.
목적 탐토ghrelin수체변화대대서술후소장동력불족적영향.방법 채용체외기조실험관찰재잡파담감(10 nmol/L)존재화결핍시,불동농도적ghrelin(0、0.01、0.1、0.5、1.0 μmol/L)대기조수축력적영향.채용면역조직화학방법화Western blot기술검측행단측장문합적실험조대서화행가수술적대조조대서소장기층ghrelin수체(GHS-R1a)적표체정황.결과 재체외,잡파담감존재시,ghrelin능구증강소장평활기기조적수축,불동농도적ghrelin(0.1、0.5、1.0 μmoL/L)인기적수축력차이구유통계학의의[(223±18)%、(245±22)%、(264±25)%,P<0.01].면역조화염색현시,GHS-R1a주요분포우소장기층,실험조대서소장배형기화종행기중GHS-R1a표체균약우대조조.Western blot결과현시,실험조소장ghrelin수체표체량(0.51±0.02)명현저우대조조(0.71±0.01,P<0.01).결론 술후소장기층ghrelin수체표체하조소도치적ghrelin효응감약가능삼여술후소장동력불족적발생.
Objective To investigate the effect of the expression of ghrelin receptors on the postoperative small intestine dysmotility in rat models.Methods The effect of different concentrations of ghrelin (0, 0.01, 0.1, 0.5, 1.0 μmol/L) on the contraction of smooth muscle strips of rat small intestine in the presence or absence of carbachol was observed in vitro. End-to-side anastomosis was performed in the study group and sham controls were used. The expression of ghrelin receptors (CHS-R1a) in small intestine muscle layers was detected by immunohistochemistry and Western blot. Results In vitro, ghrelin enhanced the contraction of smooth muscle strips in the presence of carbachol, and the differences in contraction induced by different concentrations of ghrelin(0.1, 0.5, 1.0 μmol/L) were statistically significant [(223±18)% , (245±22)% , (264±25)% , P<0.01]. Immunohistochemistry study showed that CHS-R1a mainly located in the muscular layer of the bowel wall. The expression of GHS-R1a in the circular and longitudinal muscle was significantly weaker than that in the control group. The expression of ghrelin receptors after surgery was down-regulated in the study group, which was lower than that in the control group(0.51 ±0.02 vs. 0.71±0.01, P<0.01). Conclusion Down regulation of ghrelin receptors in small intestine muscle layers may contribute to the occurrence of small intestine dysmotility after intestinal surgery.