CAJ | 학술논문

目的观察坎地沙坦对染尘大鼠矽肺形成过程中血管紧张素Ⅱ(AngⅡ)、肺纤维化及细胞因子的影响.方法将Wistar大鼠随机分为模型组、干预组、对照组,每组32只.干预组每天灌胃坎地沙坦10 mg/kg,模型组和对照组同法给予等量生理盐水;灌胃1周后,模型组和干预组水合氯醛腹腔注射麻醉,将0.5 ml矽尘混悬液(浓度为50 mg/ml)缓慢注入气管,对照组大鼠气管内注入0.5 ml灭菌生理盐水.染尘后第2天,各组大鼠恢复先前的处理后第3、7、14、28天各处死大鼠8只.计算肺脏系数并进行肺组织HE及Masson染色观察；用免疫组化方法测定肺组织血管紧张素转化酶(ACE)表达水平；用ELISA测定肺泡灌洗液中转化生长因子β1(TGF-β1)及AngⅡ含量.结果干预组大鼠第3、7、14、28天肺泡炎症和纤维化程度均较模型组明显减轻,干预组大鼠肺脏系数明显低于模型组,差异有统计学意义(P＜0.01).与对照组比较,模型组和干预组肺泡灌洗液中TGF-β1和Ang Ⅱ含量均明显升高,差异有统计学意义(P＜0.01)；与模型组比较,干预组肺泡灌洗液中TGF-β1和Ang Ⅱ含量均明显降低,差异有统计学意义(P＜0.01).模型组和干预组肺组织ACE表达明显高于对照组,干预组肺组织ACE表达明显低于模型组,差异均有统计学意义(P＜0.01).结论早期使用坎地沙坦干预能够明显减轻染尘大鼠的肺组织炎症和肺纤维化程度,降低肺泡灌洗液中的TGF-β1和AngⅡ含量,下调肺组织中ACE表达.
목적 관찰감지사탄대염진대서석폐형성과정중혈관긴장소Ⅱ(AngⅡ)、폐섬유화급세포인자적영향.방법 장Wistar대서수궤분위모형조、간예조、대조조,매조32지.간예조매천관위감지사탄10 mg/kg,모형조화대조조동법급여등량생리염수;관위1주후,모형조화간예조수합록철복강주사마취,장0.5 ml석진혼현액(농도위50 mg/ml)완만주입기관,대조조대서기관내주입0.5 ml멸균생리염수.염진후제2천,각조대서회복선전적처리후제3、7、14、28천각처사대서8지.계산폐장계수병진행폐조직HE급Masson염색관찰；용면역조화방법측정폐조직혈관긴장소전화매(ACE)표체수평；용ELISA측정폐포관세액중전화생장인자β1(TGF-β1)급AngⅡ함량.결과 간예조대서제3、7、14、28천폐포염증화섬유화정도균교모형조명현감경,간예조대서폐장계수명현저우모형조,차이유통계학의의(P＜0.01).여대조조비교,모형조화간예조폐포관세액중TGF-β1화Ang Ⅱ함량균명현승고,차이유통계학의의(P＜0.01)；여모형조비교,간예조폐포관세액중TGF-β1화Ang Ⅱ함량균명현강저,차이유통계학의의(P＜0.01).모형조화간예조폐조직ACE표체명현고우대조조,간예조폐조직ACE표체명현저우모형조,차이균유통계학의의(P＜0.01).결론 조기사용감지사탄간예능구명현감경염진대서적폐조직염증화폐섬유화정도,강저폐포관세액중적TGF-β1화AngⅡ함량,하조폐조직중ACE표체.
Objective To explore the effects of Candesartan cilexetil on the rats exposed to silica.Method Ninety-six wistar rats were randomly divided into model-group,intervention-group and control-group (32 rats a group).The intervention-group,model-group and control group were orally exposed to Candesartan cilexeti ( 10 mg/kg) and normal solution for a week,respectively.Then the model and intervention groups were exposed to silica by intratracheal infusion of silica dust suspension (50 mg/ml),the control group was exposed to 0.5 ml normal solution for 2 days.On the 3rd,7th,14th and 28th days after exposure to silica,8 rats of each group were sacrificed,respectively.The samples of lung tissues were collected.The lung/body coefficients were detected.The pathological examinations were performed by HE and Masson staining.The levels of ACE in the lung tissues were observed by immunochemistry staining.The levels of TGF-β1 and Ang Ⅱ in the BALF were examined by ELISA.Results On the 3rd,7th,14th and 28th days after exposure,the levels of alveolitis and pulmonary fibrosis in the intervention group were significantly alleviated as compared with model group,and the lung/body coefficients in the intervention group,which were significantly lower than those in model group respectively (P＜0.01).As compared with control group,the levels of TGF-β1 and Ang Ⅱ of the BALF in the model and intervention groups significantly enhanced (P＜0.01).As compared with model group,the levels of TGF-β1 and Ang Ⅱ of the BALF in the intervention group significantly decreased (P＜0.01).As compared with control group,the levels of ACE of the lung tissues in the model and interventiou groups significantly increased (P＜0.01 ).But the level of ACE of the lung tissues in the intervention group was significantly lower than that in the model group (P＜0.01).Conclusion The early Candesartan cilexetil intervention could significantly decrease the levels of alveolitis and lung fibrosis,declined the levels of TGF-β1 and Ang Ⅱ of BALF and downregulated the expression level of ACE in lung tissues in rats exposed to silica.