CAJ | 학술논문

目的探讨GSTT1、GSTM1和CYP2E1基因多态性与二甲基甲酰胺(DMF)作业人员肝脏损害的关系.方法以某皮革厂69名肝功能异常的DMF作业人员为病例组,选取与病例组相似岗位、DMF接触水平相近而肝功能正常的125名工人为对照组,应用聚合酶链反应(PCR)、PCR-RFLP方法分别对GSIT1、GSTM1和CYP2E1 PstI位点基因进行分型.结果病例组和对照组中GSTM1阳性和GSTM1阴性的分布频率分别为59.42%、38.40%和40.58%、61.60%,两组间分布的差异有统计学意义(P=0.005),携带GSTM1阳性基因型个体发生肝功能异常的风险是GSTM1阴性基因型个体的2.34倍(OR=2.34,95%CI:1.29～4.29).而GSTT1和CYP2E1 PstI位点不同基因型在病例组和对照组中没有明显差异.结论携带GSTM1阳性基因型个体的DMF作业人员发生肝功能异常的风险性可能增加.
목적 탐토GSTT1、GSTM1화CYP2E1기인다태성여이갑기갑선알(DMF)작업인원간장손해적관계.방법 이모피혁엄69명간공능이상적DMF작업인원위병례조,선취여병례조상사강위、DMF접촉수평상근이간공능정상적125명공인위대조조,응용취합매련반응(PCR)、PCR-RFLP방법분별대GSIT1、GSTM1화CYP2E1 PstI위점기인진행분형.결과 병례조화대조조중GSTM1양성화GSTM1음성적분포빈솔분별위59.42%、38.40%화40.58%、61.60%,량조간분포적차이유통계학의의(P=0.005),휴대GSTM1양성기인형개체발생간공능이상적풍험시GSTM1음성기인형개체적2.34배(OR=2.34,95%CI:1.29～4.29).이GSTT1화CYP2E1 PstI위점불동기인형재병례조화대조조중몰유명현차이.결론 휴대GSTM1양성기인형개체적DMF작업인원발생간공능이상적풍험성가능증가.
Objective To investigate abnormal liver function associated with polymorphism of GSTT1,GSTM1 and CYP2E1 in workers exposed to N,N- dimethylformamide.Methods Sixty-nine workers with abnormal liver function in a synthetic leather factory were recruited as case.One hundred and twenty five control subjects with similar work tasks were selected from the same factory.Genotypes for GSTT1 and GSTM1 were determined by multiplex PCR,and for CYP2E1 PstI by PCR-RFLP assay.Results The frequency of positive GSTM1 was 59.42% in cases and 38.40% in control,with an odds ratio (OR) of 2.34,95% CI:1.29～4.29 (P=0.005).For GSTT1 and CYP2E1 PstI,the frequencies of genotypes showed no significant difference between case and control.Conclusion GSTM1 positive genotype may be genetic risk factors for development of abnormal liver function in workers exposed to N,N- diroethylformamide.