中华胸心血管外科杂志
中華胸心血管外科雜誌
중화흉심혈관외과잡지
Chinese Journal of Thoracic and Cardiovascular Surgery
2009年
1期
41-43
,共3页
周骐%周建业%胡盛寿%胡平
週騏%週建業%鬍盛壽%鬍平
주기%주건업%호성수%호평
心肌梗死%组织工程%网膜%细胞凋亡
心肌梗死%組織工程%網膜%細胞凋亡
심기경사%조직공정%망막%세포조망
Myocardial infarction%Engineer heart tissue%Omentum%Apoptosis
目的 观察大网膜增加组织工程心肌(EHT)血供,改善微环境后对大鼠心肌梗死模型上移植细胞存活的影响.方法 结扎雌性Sprague-Dawley(SD)大鼠左冠状动脉,制作心肌梗死模型.以雄性大鼠骨髓间充质干细胞(MSCs)为种子细胞,种植于共聚乙交酯-丙交酯(poly lactic acid-CO-glycolic ac-id,PLGA)补片上构建EHT.符合心肌梗死标准的动物随机分成网膜EHT组、单纯EHT组、心肌梗死组和假手术组,每组6只鼠.组织工程心肌植入后4周,用TUNEL法检测移植细胞凋亡,标本取材做病理学检查,免疫组化方法检测内皮细胞标志物vWF表达.结果 TONEL检测发现EHT植入后1周网膜EHT组移植细胞凋亡少于单纯EHT组(P<0.05),2周、4周时两组间凋亡无明显差异(P>0.05).vWF染色发现EHT植入后1周、2周、4周网膜EHT组微血管密度均高于单纯EHT组(P<0.05).结论 网膜包裹MSCs-PLGA构建的EHT覆盖于心肌梗死表面后能够通过增加微血管密度,改善局部微环境,减少移植细胞的早期凋亡,促进移植细胞的存活.
目的 觀察大網膜增加組織工程心肌(EHT)血供,改善微環境後對大鼠心肌梗死模型上移植細胞存活的影響.方法 結扎雌性Sprague-Dawley(SD)大鼠左冠狀動脈,製作心肌梗死模型.以雄性大鼠骨髓間充質榦細胞(MSCs)為種子細胞,種植于共聚乙交酯-丙交酯(poly lactic acid-CO-glycolic ac-id,PLGA)補片上構建EHT.符閤心肌梗死標準的動物隨機分成網膜EHT組、單純EHT組、心肌梗死組和假手術組,每組6隻鼠.組織工程心肌植入後4週,用TUNEL法檢測移植細胞凋亡,標本取材做病理學檢查,免疫組化方法檢測內皮細胞標誌物vWF錶達.結果 TONEL檢測髮現EHT植入後1週網膜EHT組移植細胞凋亡少于單純EHT組(P<0.05),2週、4週時兩組間凋亡無明顯差異(P>0.05).vWF染色髮現EHT植入後1週、2週、4週網膜EHT組微血管密度均高于單純EHT組(P<0.05).結論 網膜包裹MSCs-PLGA構建的EHT覆蓋于心肌梗死錶麵後能夠通過增加微血管密度,改善跼部微環境,減少移植細胞的早期凋亡,促進移植細胞的存活.
목적 관찰대망막증가조직공정심기(EHT)혈공,개선미배경후대대서심기경사모형상이식세포존활적영향.방법 결찰자성Sprague-Dawley(SD)대서좌관상동맥,제작심기경사모형.이웅성대서골수간충질간세포(MSCs)위충자세포,충식우공취을교지-병교지(poly lactic acid-CO-glycolic ac-id,PLGA)보편상구건EHT.부합심기경사표준적동물수궤분성망막EHT조、단순EHT조、심기경사조화가수술조,매조6지서.조직공정심기식입후4주,용TUNEL법검측이식세포조망,표본취재주병이학검사,면역조화방법검측내피세포표지물vWF표체.결과 TONEL검측발현EHT식입후1주망막EHT조이식세포조망소우단순EHT조(P<0.05),2주、4주시량조간조망무명현차이(P>0.05).vWF염색발현EHT식입후1주、2주、4주망막EHT조미혈관밀도균고우단순EHT조(P<0.05).결론 망막포과MSCs-PLGA구건적EHT복개우심기경사표면후능구통과증가미혈관밀도,개선국부미배경,감소이식세포적조기조망,촉진이식세포적존활.
Objective We construct engineer heart tissue (EHT) with MSCs and PLGA. We grafted the EHT onto the surface of female myocardial infarction SD rats. We hypothesized that omentum wrapped MSCs-PLGA EHT would improve transplanted cell survival when the isehemic microenvironment was attenuated.Methods MSCs were isolated from male SD rats and cultured on PLGA polymers in vitro. Eight weeks after female SD rats were subjected to left anterior descending ligation, two treatments were conducted: MSCs-PLGA EHT implantation (group E), and omentum wrapped MSCs-PLGA EHT implantation (group O). Control group underwent no treatment (group C). The myocardial infarction group (group MI) underwent LAD ligation only. 4 weeks after EHT transplantation, the following variables were evaluated: cell apoptosis by TUNEL staining, microvascular density by immunochemistry staining of vWF, specimen staining by picrosirius red. Results Transplanted cell apoptosis was significantly reduced in group O compared with group E one week after EHT implantation (P < 0.05). Favtor Ⅷ characterized microvascular density was higher in group O compared with group E (P<0.05). Picrosirius red staining showed infarction ventricular wall thickness of group O was significantly increased compared with group MI and group C (P<0.05). Conclusion Transplantation of MSCs-PLGA EHT with omentum wrapping resulted higher microvessel density.The ameliorate microenvironment reduced cell apoptosis.