CAJ | 학술논문

为了考察鼻咽癌表达下调/缺失基因NAG11和NAG12 对鼻咽癌细胞系HNE1生长的影响 ,构建了NAG11和NAG12基因真核表达载体pcDNA3.1(+)/NAG11和pcDNA3.1(+)/NAG12,采用脂质体转染技术将真核重组质粒和空载体质粒分别导入HNE1细胞,观察转染后HNE1细胞生物学特性的变化.结果显示,NAG11重表达对HNE1细胞生长和细胞周期没有明显的影响,而NAG12 重表达对HNE1细胞有生长抑制作用,与空载体转染组相比,倍增时间由24.1 h延长至31.1 h ,停滞于G0-G1期细胞数由51.42%增加至68.14%.以上实验进一步说明鼻咽癌是多基因改变的疾病,NAG12的重表达有助于鼻咽癌恶性表型的逆转.
위료고찰비인암표체하조/결실기인NAG11화NAG12 대비인암세포계HNE1생장적영향 ,구건료NAG11화NAG12기인진핵표체재체pcDNA3.1(+)/NAG11화pcDNA3.1(+)/NAG12,채용지질체전염기술장진핵중조질립화공재체질립분별도입HNE1세포,관찰전염후HNE1세포생물학특성적변화.결과현시,NAG11중표체대HNE1세포생장화세포주기몰유명현적영향,이NAG12 중표체대HNE1세포유생장억제작용,여공재체전염조상비,배증시간유24.1 h연장지31.1 h ,정체우G0-G1기세포수유51.42%증가지68.14%.이상실험진일보설명비인암시다기인개변적질병,NAG12적중표체유조우비인암악성표형적역전.
To study the effect of NAG11 and NAG12 gene on NAG11- and NAG1 2-negat ive nasopharyngeal carcinoma (NPC) cell line HNE1, the pcDNA3.1(+)/NAG11 and pc DNA3.1(+)/NAG12 mammalial expressing recombination were constructed and were tra nfected into HNE1 cells, then cytobiologic characterization of the transfected H NE1 cells was detected by population double time (PDT) and flow cytometric analy sis. The results showed that NAG11 reexpression did not play obvious role on the cell growth of HNE1, while NAG12 reexpression had definite growth suppression on HNE1, such as a longer PDT and an accumulation of cells G1 phase. These dat a further demonstrated that NPC is a disease involving polygenic alteration and NA G12 gene could favor malignant phenotype reversion of NPC cells.